Isolation and characterization of a novel connexin gene, Cx-60, in porcine ovarian follicles

Endocrinology
K ItahanaT Takeya

Abstract

A new member of the connexin family was isolated from the porcine ovary. The amino acid sequence deduced from the nucleotide sequence of genomic as well as complementary DNA clones predicted the reading frame encoding the 60-kDa protein product, indicating the largest molecular mass among the connexin family genes analyzed to date; we named this gene Cx-60 based on its predicted molecular mass. The features of its primary structure were compared with those of other connexin genes, and the characteristics of its expression profile were examined in mammalian ovarian follicles. Cx-60 shares significant similarities with other connexins in the transmembrane and extracellular domains, but showed a highly unique primary structure in the cytoplasmic and carboxyl-terminal domains. The Cx-60 gene was unique in that its messenger RNA was detected in both the theca interna compartment and cumulus cells in the ovary; of other tissues, Cx-60 expression was relatively evident in the colon, thymus, and spleen. Cx-60, in contrast to Cx-43, was expressed constitutively upon gonadotropin stimulation when examined in hypophysectomized rats. Taken together, these results indicate that at least the connexin genes Cx-60, Cx-43, Cx-32, Cx-30.3, and C...Continue Reading

Citations

Oct 29, 2010·BMC Ophthalmology·Goran SöhlKlaus Willecke
Dec 29, 2007·Molecular and Cellular Endocrinology·Eran GershonNava Dekel
Jul 4, 2012·Reproduction in Domestic Animals = Zuchthygiene·K Knapczyk-StworaM Slomczynska
Aug 23, 2005·Biochemical and Biophysical Research Communications·Motofusa AkiyamaTatsuo Takeya
Mar 1, 2002·Biology of Reproduction·Irit GranotNava Dekel
Feb 21, 2006·Molecular Reproduction and Development·Germán G KaiserRicardo H Alberio
Mar 7, 2003·American Journal of Physiology. Cell Physiology·Joanne E I GittensGerald M Kidder
Sep 25, 2003·Physiological Reviews·Juan C SaezEric C Beyer

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