Isolation and characterization of a partial cDNA clone for heparin cofactor II1

Biochemical and Biophysical Research Communications
R C Inhorn, D M Tollefsen

Abstract

A human fetal liver cDNA library constructed in lambda gt11 was screened with affinity-purified rabbit antibodies raised against heparin cofactor II. One positive clone was plaque purified and the cDNA insert was completely sequenced. The clone encodes the C-terminal 167 amino acid residues of heparin cofactor II as well as the entire 3'-untranslated region of the message. Proline and leucine were identified in the P2 and P1 positions of the protease cleavage site, providing a possible explanation for the ability of heparin cofactor II to inhibit both thrombin and chymotrypsin-like proteases. The coding sequence is identical to that of the recently published human leuserpin 2 (Ragg (1986) Nucl. Acids Res. 14, 1073).

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Mar 1, 1989·DNA·I GaillardP Corvol
Jan 1, 1989·Annals of the New York Academy of Sciences·C W PrattF C Church
Mar 1, 1991·Blood Reviews·D N Cooper
Dec 1, 1992·Genomics·J E CollinsI Dunham
Mar 26, 2005·Science·Jill ChengThomas R Gingeras

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