Isolation and characterization of a phenol-degrading Rhodococcus sp. strain AQ5NOL 2 KCTC 11961BP

Journal of Basic Microbiology
N M ArifM Y Shukor

Abstract

In this work, we report on the isolation of a phenol-degrading Rhodococcus sp. with a high tolerance towards phenol. The isolate was identified as Rhodococcus sp. strain AQ5NOL 2, based on 16S rDNA analysis. The strain degraded phenol using the meta pathway, a trait shared by many phenol-degraders. In addition to phenol biodegradation, the strain was also capable of degrading diesel. Strain AQ5NOL 2 exhibited a broad optimum temperature for growth on phenol at between 20 °C and 35 °C. The best nitrogen sources were ammonium sulphate, glycine or phenylalanine, followed by proline, nitrate, leucine, and alanine (in decreasing efficiency). Strain AQ5NOL 2 showed a high tolerance and degradation capacity of phenol, for it was able to register growth in the presence of 2000 mg l(-1) phenol. The growth of this strain on phenol as sole carbon and energy source were modeled using Haldane kinetics with a maximal specific growth rate (μ(max)) of 0.1102 hr(-1), a half-saturation constant (K(s) ) of 99.03 mg l(-1) or 1.05 mmol l(-1), and a substrate inhibition constant (K(i)) of 354 mg l(-1) or 3.76 mmol l(-1). Aside from phenol, the strain could utilize diesel, 2,4-dinitrophenol and ρ-cresol as carbon sources for growth. Strain AQ5NOL 2 e...Continue Reading

References

Jan 1, 1990·Antonie van Leeuwenhoek·G StraubeE Straube
Dec 1, 1994·Biodegradation·J Powlowski, V Shingler
Jun 1, 1994·The Journal of Applied Bacteriology·B J JuvenH Weisslowicz
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Apr 1, 1996·Applied and Environmental Microbiology·K T Semple, R B Cain
Sep 29, 1998·Journal of Applied Microbiology·K S BellN Christofi
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·S J Traina, V Laperche
Jun 29, 2002·Applied and Environmental Microbiology·Dockyu KimEungbin Kim
Mar 17, 2004·International Journal of Systematic and Evolutionary Microbiology·Amanda L JonesMichael Goodfellow
Jun 18, 2005·Current Opinion in Biotechnology·Michael J LarkinChristopher C R Allen
Oct 6, 2005·Journal of Hazardous Materials·V ArutchelvanV Muralikrishnan
Dec 27, 2005·Bioscience, Biotechnology, and Biochemistry·San-Chin TsaiYaw-Kuen Li
Dec 1, 1995·Applied and Environmental Microbiology·G M ZaitsevM S Salkinoja-Salonen
Jun 11, 2008·Journal of Hazardous Materials·Vera Lúcia Dos SantosMarcelo Matos Santoro
Sep 18, 2008·Journal of Basic Microbiology·Riddhi Patel, Shalini Rajkumar
Feb 25, 2009·Prikladnaia biokhimiia i mikrobiologiia·E S ShumkovaL A Golovleva

❮ Previous
Next ❯

Citations

Nov 3, 2015·Marine Pollution Bulletin·Edmo M RodriguesMarcos R Tótola
Apr 28, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Linqiong WangQing Wang
Feb 12, 2020·Applied Microbiology and Biotechnology·Taisiya NoginaGeoffrey Michael Gadd
Mar 15, 2013·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Sheena KumariFaizal Bux
Aug 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yujuan WenYuesuo Yang
Dec 22, 2019·Extremophiles : Life Under Extreme Conditions·Ahmad Fareez Ahmad RosleeSiti Aqlima Ahmad
Dec 2, 2020·Environmental Pollution·Edmo Montes RodriguesMarcos Rogério Tótola

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.