PMID: 8589426Jan 1, 1996Paper

Isolation and characterization of a pSym locus of Rhizobium sp. BR816 that extends nodulation ability of narrow host range Phaseolus vulgaris symbionts to Leucaena leucocephala

Molecular Plant-microbe Interactions : MPMI
P van RhijnJ Vanderleyden

Abstract

Introduction of a cosmid library of megaplasmid DNA of Rhizobium sp. BR816, a broad host range Rhizobium strain, into R. etli CE3, a narrow host range bean symbiont, resulted in the isolation of a transconjugant that could effectively nodulate Leucaena leucocephala. Analysis of the corresponding cosmid, pBRF2, revealed the presence of genes required for elicting nitrogen-fixing nodules on L. leucoephala. Subcloning and Tn5 tagging identified a locus responsible for the host range extension. Sequence analysis of this locus revealed an ORF that shows significant identity with NodO of R. leguminosarum bv. viciae.

Citations

Jul 5, 2001·Molecular Plant-microbe Interactions : MPMI·E LuytenJ Vanderleyden
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·X PerretW J Broughton
Sep 24, 1999·Biochemical and Biophysical Research Communications·M D SerraG Menestrina
Jul 12, 2008·FEMS Microbiology Letters·Maarten Fauvart, Jan Michiels
Feb 12, 2014·FEMS Microbiology Ecology·Ravindra Pal Singh, C R K Reddy
Mar 30, 2007·International Journal of Systematic and Evolutionary Microbiology·José Luis Zurdo-PiñeiroEncarna Velázquez
Jul 10, 2014·BMC Genomics·Marco A RogelEsperanza Martínez-Romero

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