Isolation and characterization of a rolling-circle-type plasmid from Rhodococcus erythropolis and application of the plasmid to multiple-recombinant-protein expression

Applied and Environmental Microbiology
Nobutaka Nakashima, Tomohiro Tamura

Abstract

We isolated, sequenced, and characterized the cryptic plasmid pRE8424 from Rhodococcus erythropolis DSM8424. Plasmid pRE8424 is a 5,987-bp circular plasmid; it carries six open reading frames and also contains cis-acting elements, specifically a single-stranded origin and a double-stranded origin, which are characteristic of rolling-circle-replication plasmids. Experiments with pRE8424 derivatives carrying a mutated single-stranded origin sequence showed that single-stranded DNA intermediates accumulated in the cells because of inefficient conversion from single-stranded DNA to double-stranded DNA. This result indicates that pRE8424 belongs to the pIJ101/pJV1 family of rolling-circle-replication plasmids. Expression vectors that are functional in several Rhodococcus species were constructed by use of the replication origin from pRE8424. We previously reported a cryptic plasmid, pRE2895, from R. erythropolis, which may replicate by a theta-type mechanism, like ColE2 plasmids. The new expression vectors originating from pRE8424 were compatible with those derived from pRE2895. Coexpression experiments with these compatible expression vectors indicated that the plasmids are suitable for the simultaneous expression of multiple recom...Continue Reading

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Citations

Feb 21, 2006·Applied Microbiology and Biotechnology·Raku ShinkyoToshiyuki Sakaki
Feb 14, 2008·Applied Microbiology and Biotechnology·Kohsuke HondaHisao Ohtake
Mar 6, 2012·Applied Microbiology and Biotechnology·Junji InokoshiHiroshi Tomoda
Aug 29, 2012·Applied Microbiology and Biotechnology·Martín A HernándezHéctor M Alvarez
Oct 16, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Laura Fernández de Las HerasJuana María Navarro Llorens
Apr 18, 2012·Journal of the American Chemical Society·Atsushi MinamiHideaki Oikawa
May 8, 2010·The Journal of Biological Chemistry·Carola DresenLindsay D Eltis
Jun 19, 2010·The Journal of Biological Chemistry·Sachi OkamotoLindsay D Eltis
Apr 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yoshiaki YasutakeTomohiro Tamura
Oct 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yu MatsumotoMasaru Wada
Jul 18, 2012·Antimicrobial Agents and Chemotherapy·Peter SpanogiannopoulosGerard D Wright
Feb 14, 2012·Applied and Environmental Microbiology·Ariel GrosternLisa Alvarez-Cohen
Feb 16, 2010·Applied and Environmental Microbiology·Khalid Ibrahim SallamTomohiro Tamura
Nov 8, 2011·Applied and Environmental Microbiology·Hao-Ping ChenLindsay D Eltis
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Mar 25, 2005·Microbial Cell Factories·Nobutaka NakashimaTomohiro Tamura
Oct 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hea-Yeon YunTomohiro Tamura
Oct 8, 2013·Applied and Environmental Microbiology·Christopher M SalesLisa Alvarez-Cohen
Feb 2, 2016·Bioscience, Biotechnology, and Biochemistry·Yuki AtagoHirofumi Hara
May 9, 2012·Journal of Bioscience and Bioengineering·Nobutaka Nakashima, Tomohiro Tamura
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Aug 7, 2007·Biochemical and Biophysical Research Communications·Yoshiaki YasutakeTomohiro Tamura
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Aug 19, 2005·Journal of Biotechnology·Khalid Ibrahim SallamTomohiro Tamura
Dec 17, 2014·Applied Microbiology and Biotechnology·Harini VenkataramanLubbert Dijkhuizen
Jul 18, 2006·FEMS Microbiology Letters·Meijuan ShenZhongjun Qin
Nov 9, 2006·Bioscience, Biotechnology, and Biochemistry·Woan-Sub KimTomohiro Tamura
Apr 30, 2016·Scientific Reports·Sawsan AmaraLindsay D Eltis

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