Isolation and characterization of a strain of Lichtheimia corymbifera (ex Absidia corymbifera) from a case of bovine abortion.

Reproductive Biology and Endocrinology : RB&E
Chiara PiancastelliClotilde S Cabassi

Abstract

Lichtheimia corymbifera (previously Absidia corymbifera) is a filamentous zygomycetes belonging to the order Mucorales and to the family Lichtheimiaceae. Members of genus Lichtheimia spp. are cosmopolitan and ubiquitous in nature. Lichtheimia corymbifera is a recognized agent of diseases in man and animals. In cattle it causes abortion and mastitis. Three cases of bovine abortion occurred in a herd located in the Po Valley. Serological examinations were performed on fetal and mother's blood. One of the aborted fetus was referred to our laboratory. The paper describes the isolation and characterization of Lichtheimia corymbifera from a bovine aborted fetus. Serological examinations were performed on fetal and mother's blood. Lesions on fetal tissues and placenta leaded the diagnostic suspect towards a mycotic aetiology. Tissues were then put in culture, and at the same time an histological examination was performed, together with bacteriological and virological tests. The isolate from placenta and fetal tissues was identified and characterized by PCR and RFLP, using the ITS region as a target sequence and AclI restriction site within the amplicon to distinguish Lichtheimia corymbifera among the other fungi. Serological, bacterio...Continue Reading

References

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Citations

Aug 1, 2014·Current Microbiology·Wieland SchrödlMonika Krüger
Sep 5, 2014·Mycoses·Volker U Schwartze, Ilse D Jacobsen
Jun 11, 2016·Jundishapur Journal of Microbiology·Ardeshir ZiaeeJamal Hashemi
Apr 18, 2020·The Veterinary Record

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Methods Mentioned

BETA
periodic acid Schiff
ELISA
PCR
Electrophoresis

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