PMID: 8938373Feb 1, 1996Paper

Isolation and characterization of a subtilisin-like serine proteinase secreted by the sap-staining fungus Ophiostoma piceae

Enzyme and Microbial Technology
L D Abraham, C Breuil

Abstract

Identification of key enzymes of sapstain fungi which cause wood discoloration is necessary for targeted inhibition strategies. Therefore proteinases involved in the nitrogen pathway have been characterized. The sap-staining fungus Ophiostoma piceae strain 387N produced proteolytic enzymes when grown on wood and protein-supplemented media. Proteolytic activity in culture filtrates was inhibited by PMSF and EDTA. The major protein in culture filtrates was a proteinase with a pI of 5.6 and a molecular weight of 33 kDa. This was the major proteinase produced by O. piceae and it was purified from culture filtrates by hydrophobic interaction chromatography. The proteinase was susceptible to autolytic degradation during chromatographic separations when ammonium sulfate was not present. When azocoll was used as a substrate, the proteolytic activity of the purified proteinase was determined to be optimal at pH 7-9 and 40 degrees C. Similar pH and temperature optima were obtained using succinyl-ala-ala-pro-phe-p-nitroanilide as the substrate. The N-terminal sequence of the protein showed a high degree of homology with fungal alkaline serine proteinases classified as subtilisin class II enzymes. Agreement in inhibition patterns and elect...Continue Reading

References

Mar 11, 1992·Infection and Immunity·M FroscoJ D Macmillan
Feb 15, 1991·Analytical Biochemistry·A L CheungV A Fischetti
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1989·Chemical & Pharmaceutical Bulletin·C BarthomeufA Pourrat
Aug 1, 1988·Trends in Biochemical Sciences·J A Wells, D A Estell
Feb 1, 1984·Analytical Biochemistry·R ChaviraJ H Hageman
Jan 1, 1982·Methods in Enzymology·K J Ellis, J F Morrison
Jun 1, 1993·Infection and Immunity·P E KolattukudyE A Copelan
Jan 1, 1960·Annual Review of Biochemistry·B S HARTLEY
Jan 1, 1977·Applied and Environmental Microbiology·B S Montenecourt, D E Eveleigh
Dec 1, 1984·Applied and Environmental Microbiology·I DillG Kraepelin
Sep 1, 1991·Applied and Environmental Microbiology·H Laan, W N Konings

❮ Previous
Next ❯

Citations

Apr 5, 2008·Canadian Journal of Microbiology·Alexandre José MacedoCarlos Termignoni
Mar 19, 2013·BMC Genomics·Shima KhoshraftarDinesh Christendat
May 5, 2000·Molecular Plant-microbe Interactions : MPMI·A J CarlileR M Cooper
Nov 20, 2012·Journal of Economic Entomology·N S LittleJ J Riggins
Oct 26, 2014·Mycologia·Michael Stefan MayerhoferGavin Kernaghan Kernaghan
Nov 5, 2014·World Journal of Microbiology & Biotechnology·Ravindranathan Aswati Nair, Chellappan Geethu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.