Isolation and characterization of a symbiosis-regulated ras from the ectomycorrhizal fungus Laccaria bicolor

Molecular Plant-microbe Interactions : MPMI
S SundaramG K Podila

Abstract

Ectomycorrhizae formed by the symbiotic interaction between ectomycorrhizal fungi and plant roots play a key role in maintaining and improving the health of a wide range of plants. Mycorrhizal initiation, development, and functional maintenance involve morphological changes that are mediated by activation and suppression of several fungal and plant genes. We identified a gene, Lbras, in the ectomycorrhizal fungus Laccaria bicolor that belongs to the ras family of genes, which has been shown in other systems to be associated with signaling pathways controlling cell growth and proliferation. The Lbras cDNA complemented ras2 function in Saccharomyces cerevisiae and had the ability to transform mammalian cells. Expression of Lbras, present as a single copy in the genome, was dependent upon interaction with host roots. Northern analysis showed that expression was detectable in L bicolor 48 h after interaction as well as in the established mycorrhizal tissue. Phylogenetic analysis with other Ras proteins showed that Lbras is related most closely to Aras of Aspergillus nidulans.

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Citations

Jun 7, 2005·Journal of Microbiological Methods·Aída V Rodríguez-TovarBeatriz Xoconostle-Cázares
Aug 16, 2002·Current Opinion in Plant Biology·Verena Wiemken, Thomas Boller
Feb 18, 2004·Molecular Plant-microbe Interactions : MPMI·Tomas JohanssonAnders Tunlid
Sep 24, 2004·Molecular Plant-microbe Interactions : MPMI·Jean-Philippe CombierGilles Gay
May 24, 2012·Biotechnology & Genetic Engineering Reviews·Marjatta RaudaskoskiJ Stephen Horton
Jun 14, 2005·The New Phytologist·Laura MiozziPaola Bonfante
May 1, 2021·Cells·Mitzuko Dautt-CastroSergio Casas-Flores
Jun 1, 2002·The New Phytologist·Gopi K Podila
Feb 1, 2004·The New Phytologist·Peter J Lammers

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