Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B.

Journal of Bacteriology
H Itikawa, J Ryu

Abstract

A temperature-sensitive dnaK mutant (strain MT112) was isolated from Escherichia coli B strain H/r30RT by thymineless death selection at 43 degrees C. By genetic mapping, the mutation [dnaK7(Ts)] was located near the thr gene (approximately 0.2 min on the may). E. coli K-12 transductants of the mutation to temperature sensitivity were assayed for their susceptibility to transducing phage lambda carrying the dnaK and/or the dnaJ gene. All of the transductants were able to propagate phage lambda carrying the dnaK gene. When macromolecular synthesis of the mutant was assayed at 43 degrees C, it was observed that both deoxyribonucleic acid and ribonucleic acid syntheses were severely inhibited. Thus, it was suggested that the conditionally defective dnaK mutation affects both cellular deoxyribonucleic acid and ribonucleic acid syntheses at the nonpermissive temperature in addition to inability to propagate phage lambda at permissive temperature.

References

Feb 28, 1977·Molecular & General Genetics : MGG·M SunshineJ Yochem
Feb 28, 1977·Molecular & General Genetics : MGG·C P Georgopoulos
Jun 15, 1977·Journal of Molecular Biology·H Saito, H Uchida
Aug 4, 1978·Molecular & General Genetics : MGG·H Saito, H Uchida
Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
May 25, 1975·Journal of Molecular Biology·R D'AriM B Yarmolinsky
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·M Kohiyama
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·Y HirotaF Jacob

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Citations

Jan 1, 1980·Molecular & General Genetics : MGG·C P GeorgopoulosM Feiss
Aug 2, 2011·Biotechnology Letters·Adélle BurgerAileen Boshoff
Jul 17, 2012·Applied and Environmental Microbiology·Zachary L PrattCharles W Kaspar
Jan 25, 2012·Biochemical Genetics·Swati Saha, Tarakdas Basu
Jan 1, 1985·CRC Critical Reviews in Biochemistry·E A Craig
Dec 19, 2006·Journal of Bacteriology·José A LemosRobert A Burne
Nov 19, 2014·Antimicrobial Agents and Chemotherapy·Ken-Ichi Arita-MoriokaShinya Sugimoto
Feb 13, 2019·Cold Spring Harbor Perspectives in Biology·Zlata GvozdenovBrian C Freeman
May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Nov 1, 1986·Journal of Bacteriology·S L GomesA M Silva
Apr 1, 1986·Molecular and Cellular Biology·K B PalterE A Craig
Dec 1, 1987·Journal of Bacteriology·M PatoA Toussaint
Apr 20, 1987·Journal of Molecular Biology·S H Reuter, L Shapiro
Jul 29, 2009·Biochimica Et Biophysica Acta·Daniela BressaninAlejandro Hochkoeppler

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