Isolation and characterization of a temperature-sensitive circadian clock mutant of Neurospora crassa.

Genetics
Louis W Morgan, J F Feldman

Abstract

A new circadian clock mutant has been isolated in Neurospora crassa. This new mutation, called period-6 (prd-6), has two features novel to known clock mutations. First, the mutation is temperature sensitive. At restrictive temperatures (above 21 degrees) the mutation shortens circadian period length from a wild-type value of 21.5 hr to 18 hr. At permissive temperatures (below 21 degrees) the mutant has a 20.5-hr period length close to that of the wild-type strain. Second, the prd-6 mutation is epistatic to the previously isolated clock mutation period-2 (prd-2). This epistasis is unusual in that the prd-2 prd-6 double mutant strain has an 18-hr period length at both the restrictive and permissive temperatures. That is, the temperature-sensitive aspect of the phenotype of the prd-6 strain is lost in the prd-2 prd-6 double mutant strain. This suggests that the gene products of the prd-2 and prd-6 loci may interact physically and that the presence of a normal prd-2+ protein is required for low temperature to "rescue" the prd-6 mutant phenotype. These results, combined with our recent finding that prd-2 and some alleles of the frq gene show genetic synergy, suggest that it may be possible to establish a more comprehensive model of ...Continue Reading

Citations

Feb 26, 2000·Trends in Genetics : TIG·P L Lakin-Thomas
Feb 13, 1999·Cell·J C Dunlap
Mar 6, 1999·Current Opinion in Microbiology·J J Loros
Apr 1, 2016·Journal of Biological Rhythms·Keyur AdhvaryuPatricia Lakin-Thomas
Nov 17, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·L W MorganD Bell-Pedersen
Feb 22, 2001·Annual Review of Physiology·J J Loros, J C Dunlap
May 7, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J C DunlapS K Crosthwaite
Sep 8, 1998·Current Opinion in Genetics & Development·J C Dunlap
May 21, 2010·Plant Signaling & Behavior·Angelica Lindlöf
Mar 1, 2011·Fungal Biology·Michael K WattersErik Lindamood

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