Isolation and characterization of a trypsin inhibitor from the seeds of kohlrabi (Brassica napus var. rapifera) belonging to the napin family of storage proteins

Carlsberg Research Communications
I SvendsenN Genov

Abstract

A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined by amino acid sequence analysis which also showed the inhibitor to be polymorphous. The inhibitor has been further characterized by means of molecular weight determination using SDS gel-electrophoresis and by amino acid analysis, fluorimetry as well as circular dichroism. A simplified method for purification of napins is given.

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Citations

Nov 14, 2000·Plant Science : an International Journal of Experimental Plant Biology·R WijayaG M Polya
Nov 16, 2004·Critical Reviews in Food Science and Nutrition·E N Clare MillsPeter R Shewry
Aug 1, 1995·Biological Reviews of the Cambridge Philosophical Society·P R Shewry
Aug 11, 2001·Journal of Enzyme Inhibition·J VioqueF Millán
Nov 11, 2005·The FEBS Journal·John T Christeller
Nov 1, 1993·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·G A StewartA S McWilliam
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·P R ShewryN G Halford

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