PMID: 2579156Apr 1, 1985Paper

Isolation and characterization of a tumor-specific T suppressor factor from a T cell hybridoma

The Journal of Immunology : Official Journal of the American Association of Immunologists
J K SteeleJ G Levy

Abstract

In our laboratory we have described a monoclonal antibody, B16G, which has been shown to bind to suppressive T cell factors (TsF) in DBA/2 mice. Therefore, B16G was used as a probe to identify T cell hybridomas secreting putative TsF. Hybridomas were obtained by the fusion of DBA/2 thymocytes stimulated in vivo by P815 tumor membrane extracts with the thymoma BW5147. One such hybridoma, A10, was selected and used for additional studies. From both the supernatants and ascites fluid of this hybrid a factor could be obtained that could specifically bind to both B16G and P815 antigen immunoadsorbent columns, and that scored positively with B16G in an ELISA after elution. Such reactivity could not be obtained from A10 supernatants or ascites absorbed over irrelevant columns, nor was it obtained from supernatants or ascites from other T cell hybrids that had scored B16G nonreactive in the original screening. In vivo studies indicated that affinity-purified A10 material injected into DBA/2J mice enhanced significantly the growth of P815 tumor cells, but not the growth of other DBA/2 syngeneic tumor lines such as L1210 or M-I. Additionally, this material did not inhibit the in vitro mixed leukocyte reaction (MLR) between DBA/2 splenocy...Continue Reading

Related Concepts

Monoclonal Antibodies
Antigenic Specificity
Tumor Antigens
Binding Sites, Antibody
Hybridomas
Leukemia L1210
Lymphokines
Mice, Inbred DBA
Neoplasms, Experimental
Sarcoma, Jensen

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