PMID: 3768903Aug 15, 1986Paper

Isolation and characterization of a wheat germ agglutinin-binding glycoprotein from B16 mouse melanoma cells

Carbohydrate Research
P D PinnaduwageE A Davidson

Abstract

Cells of B16 mouse melanoma grown in serum-free medium in the presence of [3H]glucosamine secreted or shed labeled glycoproteins. A wheat germ agglutinin-binding glycoprotein was isolated that accounted for 37% of the total [3H]glucosamine incorporated; it had a molecular weight of approximately 50,000 and was absent in less-tumorgenic wheat germ agglutinin (isolectin I)-resistant variants of the cells. The glycoprotein contained approximately 25% of serine and threonine plus equimolar amounts of glucosamine and galactosamine, indicating both N- and O-linked oligosaccharide chains. Neuraminidase treatment released approximately 60% of the glycoprotein's 3H radioactivity as N-acetylneuraminic acid. The sialoglycoprotein did not, but the desialylated species did, bind (97%) to ricin-Sepharose, suggesting the presence of terminal sialic acid and penultimate galactose residues in most of the saccharide units. Alkaline borohydride released 61% of the glycoprotein's radioactivity as oligosaccharide alcohols that were mainly tetrasaccharides (75%) with some branched trisaccharides (10%) from the O-linked structures. Hydrazinolysis and analysis of the alkaline borohydride-resistant portion of the glycoprotein indicated the presence of ...Continue Reading

References

Dec 11, 1979·Biochemistry·E V Chandrasekaran, E A Davidson
Aug 1, 1977·Carbohydrate Research·V P Bhavanandan, E A Davidson
May 7, 1976·Analytical Biochemistry·M TomanaS Porch
May 3, 1976·Biochemical and Biophysical Research Communications·V P Bhavanandan, E A Davidson
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H P Schnebli, M M Burger
Jan 1, 1974·Methods in Enzymology·Y NagataM M Burger
Mar 12, 1974·Biochemistry·C SatoE A Davidson
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Feb 12, 1980·Biochemical and Biophysical Research Communications·S TakasakiA Kobata
Jan 1, 1980·Advances in Carbohydrate Chemistry and Biochemistry·J Montreuil
Jan 1, 1982·Methods in Enzymology·S TakasakiA Kobata
Mar 3, 1981·Biochemistry·S D Bolmer, E A Davidson
Apr 1, 1981·Analytical Biochemistry·J U Baenziger, M Natowicz
Aug 1, 1981·Carbohydrate Research·M L BerghD H van den Eijnden
Jul 1, 1981·Analytical Biochemistry·S J Mellis, J U Baenziger
Jun 1, 1981·The Biochemical Journal·K YamamotoT Osawa
Feb 1, 1980·Analytical Biochemistry·D A Blake, I J Goldstein
Feb 1, 1980·Archives of Biochemistry and Biophysics·E LiS Kornfeld
Jan 1, 1953·Advances in Cancer Research·R J WINZLER
Oct 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·H R CATCHPOLE

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.