PMID: 11310567Apr 20, 2001Paper

Isolation and characterization of a xylose-glucose isomerase from a new strain Streptomyces thermovulgaris 127, var. 7-86

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
V RaykovskaW Voelter

Abstract

A thermostable D-xylose-glucose isomerase was isolated from the thermophilic strain Streptomyces thermovulgaris 127, var. 7-86, as a result of mutagenic treatment by gamma-irradiation of the parent strain, by precipitation and sequential chromatographies on DEAE-Sephadex A50, TSK-gel, FPLC-Mono Q/HR, and Superose 12 columns. The N-terminal amino acid sequence and amino acid analysis shows 73-92% homology with xylose-glucose isomerases from other sources. The native molecular mass, determined by gel filtration on a Superose 12 column, is 180 kDa, and 44.6 and 45 kDa were calculated, based on amino acid analysis and 10% SDS-PAGE, respectively. Both, the activity and stability of the enzyme were investigated toward pH, temperature, and denaturation with guanidine hydrochloride. The enzyme activity showed a clear pH optimum between pH 7.2 and 9.0 with D-glucose and 7.4 and 8.3 with D-xylose as substrates, respectively. The enzyme is active up to 60-85 degrees C at pH 7.0, using D-glucose, and up to 50-60 degrees C at pH 7.6, using D-xylose as substrates. The activation energy (Ea = 46 kJ x mol(-1)) and the critical temperature (Tc = 60 degrees C) were determined by fluorescence spectroscopy. Tc is in close coincidence with the melt...Continue Reading

References

Feb 1, 1992·Bioscience, Biotechnology, and Biochemistry·W BasukiN Minamiura
Sep 5, 1989·Biochemistry·G K FarberG A Petsko
Oct 11, 1988·Nucleic Acids Research·D DrocourtG Tiraby
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C ChangS W Suh
Mar 29, 2000·The International Journal of Biochemistry & Cell Biology·P Dolashka-AngelovaW Voelter
Jan 1, 1994·World Journal of Microbiology & Biotechnology·L FrioniH G Diem

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