PMID: 7076649Mar 1, 1982

Isolation and characterization of alkali-labile oligosaccharide units from porcine erythrocyte glycophorin

Journal of Biochemistry
I KawashimaA Hamada

Abstract

The oligosaccharide units of glycophorin isolated from porcine erythrocyte membranes were released by alkaline borohydride treatment and purified by gel filtration and ion-exchange chromatography. Structures of the O-glycosidic oligosaccharides were determined by methylation analysis, the methylated sugar being identified by gas-liquid chromatography-mass spectrometry and nitrous acid deamination after hydrazinolysis. The major oligosaccharide was a trisaccharide, Gal(1 leads to 3)[Neu-NGly(2 leads to 6)]GalNAc. The other oligosaccharides were larger and contained Glc-NAc. One was a pentasaccharide, Gal(1 leads to 3)Gal(1 leads to 4)GlcNAc(1 leads to 3)Gal(1 leads to 3)GalNAc. The structure of the trisaccharide was also analyzed by direct-probe mass spectrometry of the permethylated derivative, and the result obtained was consistent with the proposed structure.

Related Concepts

Carbohydrate Linkage
Carbohydrate Sequence
Avazyme
Red Cell Ghost
Glycopeptides
Ss Erythrocyte Membrane Sialoglycoproteins
Methylation
Oligosaccharides
Sialoproteins
Mass Spectrometry

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