PMID: 7390957Mar 1, 1980

Isolation and characterization of alkali-labile oligosaccharide units from horse glycophorin

Journal of Biochemistry
K FukudaA Hamada

Abstract

The O-glycosidically linked carbohydrate units of glycophorin derived from horse erythrocyte membrane were released as reduced oligosaccharides by alkaline borohydride treatment. One tetrasaccharide, two trisaccharides, and two disaccharides were purified by gel filtration and ion-exchange chromatography. Studies employing periodate oxidation, methylation analysis and gas-liquid chromatography-mass spectrometry revealed the following structures for these oligosaccharides; a tetrasaccharide; N-glycolylneuraminyl-(2 leads to 3)-D-galactopyranosyl-(1 leads to 3)-[N-glycolylneuraminyl-(2 leads to 6)]-D-N-acetylgalactosaminitol, trisaccharides; N-glycolylneuraminyl-(2 leads to 3)-D-galactopyranosyl-(1 leads to 3)-D-N-acetylgalactosaminitol and D-galactopyranosyl-(1 leads to 3)-[N-glycolylneuraminyl-(2 leads to 6)-a1-D-N-acetylgalactosaminitol, disaccharides; D-galactopyranosyl-(1 leads to 3)-D-N-acetylgalactosaminitol and N-glycolylneuraminyl-(2 leads to 3)-D-galacitol.

Citations

Apr 1, 1982·Acta Physiologica Scandinavica·E Bojesen, I N Bojesen
Mar 3, 2010·Rapid Communications in Mass Spectrometry : RCM·Enriqueta CasalJesús Eduardo Quintanilla-López
Feb 18, 2011·Canadian Journal of Microbiology·Masato ImaseHideki Aoyagi
Mar 1, 1982·European Journal of Biochemistry·M DukK Waśniowska

Related Concepts

Alkalies
Red Cell Ghost
Ss Erythrocyte Membrane Sialoglycoproteins
Oligosaccharides
Sialoproteins

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