Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.)

Journal of Plant Physiology
Maria H Cruz de CarvalhoYasmine Zuily Fodil

Abstract

A cowpea (Vigna unguiculata cv. EPACE-1) aspartic proteinase (AP) gene was isolated by genomic Library screening. Sequence analysis shows that this AP gene follows the same pattern of intron/exon number and organization as the other isolated plant AP genes, which are distinct from other solved AP genes. Northern blot analysis revealed that cowpea AP accumulates in leaves and stems but not in roots, indicating tissue-specific expression. An increased accumulation of transcripts during senescence suggests enzyme involvement in this process.

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Citations

Nov 14, 2000·Plant Science : an International Journal of Experimental Plant Biology· Cruz de Carvalho MH Tran Thanh Van K
Aug 14, 2010·Journal of Plant Physiology·Dominique Contour-AnselMaria Helena Cruz de Carvalho
Aug 1, 2009·Journal of Plant Physiology·Gordana S TimotijevićVesna R Maksimović
Apr 17, 2007·The FEBS Journal·Catarina PimentelClaudina Rodrigues-Pousada
Jan 25, 2012·International Journal of Molecular Sciences·Miroljub BaraćNikola Ristić

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