PMID: 6769923Jun 25, 1980

Isolation and characterization of an endo-beta-galactosidase from a new strain of Escherichia freundii.

The Journal of Biological Chemistry
H NakagawaY T Li

Abstract

A new strain of Escherichia freundii was isolated from human feces. Compared with the previous strain (Kitamikado, M., and Ueno, R. (1970) Bull. Jpn. Soc. Sci. Fish. 36, 1175-1180), this strain releases comparable levels of endo-beta-galactosidase with lower levels of exoglycosidases in the culture medium containing 0.3% of keratan sulfate. Endo-beta-galactosidase was purified by an improved purification procedure involving Amicon H1P10 hollow fiber filtration, QAE-Sephadex A-50, and CM-Sephadex C-50 chromatographies. The purified enzyme is completely free from proteases and exoglycosidases. The general properties of this enzyme are: molecular weight, 28,000; optimal pH, 5.5 TO 5.8; PI, pH 8.0. This enzyme hydrolyzed keratan sulfates isolated from different sources to produce 6-O-sulfo-GlcNAc beta1 leads to 3Gal as the major product. In addition, the specificity of this enzyme toward various glycoconjugates was also studied.

Related Concepts

Metazoa
Lactogest
Carbohydrate Linkage
Escherichia
Feces
Galactosidase
Glycosphingolipids
Homo sapiens
Milk (body substance)
Molecular Weight

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