PMID: 37915Jul 11, 1979

Isolation and characterization of an enkephalin-degrading aminopeptidase from rat brain

Biochimica Et Biophysica Acta
H P SchnebliR K Barclay


An enkephalin-degrading aminopeptidase from rat brain extracts has been purified to apparent homogeneity. This enzyme cleaves the N-terminal tyrosine from Leu-enkephalin and hydrolyzes some beta-naphthylamides and p-nitro-anilides of neutral, basic and aromatic, but not acidic, amino acids. The enzyme requires a free amino group on the substrate and has a neutral pH optimum. After dialysis against EDTA, the enzyme requires a divalent cation (Zn2+, Co2+ greater than Mn2% greater than Mg2+) for activity. The enzyme is inhibited by puromycin, o-phenanthroline, p-chloromercuribenzoate and EDTA, but not by puromycin, methylsulfonyl fluoride or a specific peptide inhibitor of leucine amino-peptidase. The aminopeptidase consists of two subunits and has a molecular weight of about 100 000.


Feb 1, 1989·Biulleten' eksperimental'noĭ biologii i meditsiny·A S Kamysheva
Aug 5, 1983·European Journal of Pharmacology·M AltsteinS Blumberg
Nov 1, 1983·Pharmacology, Biochemistry, and Behavior·K R DavisR Wolfenden
Jan 1, 1981·Pharmacology & Therapeutics·M E Reith, A Neidle
Jan 1, 1981·Peptides·K S HuiA Lajtha
Jan 1, 1982·Progress in Neurobiology·R C Frederickson, L E Geary
Dec 1, 1980·Neuroscience Letters·K S HuiA Lajtha
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·A N de SouzaK M Carvalho
Feb 19, 2002·Neuroscience Research·Masaru YamamotoTakeshi Kato
Feb 13, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·María Jesús Ramírez-ExpósitoM Ramírez
Apr 27, 1999·FEBS Letters·A El Khissiin, G Leclercq
Nov 5, 2002·Cell Biochemistry and Function·María Jesús Ramírez-ExpósitoJ M Martínez-Martos
Dec 9, 2009·Antiviral Research·Ulrika TehlerGordon L Amidon
Mar 30, 1982·Biochemical and Biophysical Research Communications·T HazatoT Yamamoto
Jul 31, 1980·Biochemical and Biophysical Research Communications·M Benuck, N Marks
May 1, 1992·Archives of Biochemistry and Biophysics·Y HiroiY Natori
Dec 1, 1983·Neuropeptides·N W BunnettJ H Walsh
Apr 1, 1987·Biochemical Medicine and Metabolic Biology·Y WatanabeY Fujimoto
Jun 1, 1980·Pharmacological Research Communications·L J TraficanteS Gershon
Feb 1, 1990·Archives of Biochemistry and Biophysics·G D Johnson, L B Hersh
May 1, 1985·Biochemical Pharmacology·A J TurnerA J Kenny
Aug 1, 1997·Archives of Biochemistry and Biophysics·H Erbeznik, L B Hersh
Oct 31, 1980·Biochemical and Biophysical Research Communications·R K Barclay, M A Phillipps
May 20, 2003·Biological Chemistry·Alicja BukowskaUwe Lendeckel
Jan 1, 1984·Journal of Neuroscience Research·M J Berg, N Marks
Jan 1, 1985·Annals of the New York Academy of Sciences·M Deschodt-Lanckman
Jan 1, 1989·Journal of Enzyme Inhibition·P S WilliamsJ P Gonzalez
Aug 4, 2004·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·María Dolores MayasJosé Manuel Martínez-Martos


Feb 1, 1977·Neuropharmacology·J L MeekE Costa
Apr 28, 1978·Biochemical and Biophysical Research Communications·R K Barclay, M A Phillips
Dec 15, 1973·Biochemical Pharmacology·J A GuimarãesJ L Prado
May 1, 1974·Journal of Neurochemistry·N MarksA Lajtha
Oct 29, 1971·Brain Research·N Marks, M Pirotta
Jan 1, 1965·Annual Review of Biochemistry·M L BENDER, J KEZDY

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