Isolation and characterization of an ether-type polyurethane-degrading micro-organism and analysis of degradation mechanism by Alternaria sp

Journal of Applied Microbiology
Y MatsumiyaM Kubo

Abstract

To degrade ether-type polyurethane (ether-PUR), ether-PUR-degrading micro-organism was isolated. Moreover, ether-PUR-degrading mechanisms were analysed using model compounds of ether-PUR. A fungus designated as strain PURDK2, capable of changing the configuration of ether-PUR, has been isolated. This isolated fungus was identified as Alternaria sp. Using a scanning electron microscope, the grid structure of ether-PUR was shown to be melted and disrupted by the fungus. The degradation of ether-PUR by the fungus was analysed, and the ether-PUR was degraded by the fungus by about 27.5%. To analyse the urethane-bond degradation by the fungus, a degraded product of ethylphenylcarbamate was analysed using GC/MS. Aniline and ethanol were detected by degradation with the supernatant, indicating that the fungus secreted urethane-bond-degrading enzyme(s). PURDK2 also degraded urea bonds when diphenylmethane-4,4'-dibutylurea was used as a substrate. The enzyme(s) from PURDK2 degraded urethane and urea bonds to convert the high molecular weight structure of ether-PUR to small molecules; and then the fungus seems to use the small molecules as an energy source. Ether-PUR-degrading fungus, strain PURDK2, was isolated, and the urethane- and ur...Continue Reading

References

Dec 1, 1991·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·B JansenG Pulverer
Jan 1, 1980·Journal of the American Academy of Dermatology·W B Shelley, M G Wood
Dec 1, 1994·Applied and Environmental Microbiology·S CamareroA T Martínez
Oct 1, 1994·Journal of Biomedical Materials Research·J P SanterreG A Adams
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·B Van den BurgV G Eijsink
Mar 26, 1999·Applied Microbiology and Biotechnology·T Nakajima-KambeT Nakahara
May 27, 2003·Bioresource Technology·C S K ReddyV C Kalia
Jul 26, 2005·Applied Microbiology and Biotechnology·Yukie Akutsu-ShigenoToshiaki Nakajima-Kambe
Oct 20, 2005·Journal of Bioscience and Bioengineering·D KomaM Kubo
Dec 14, 2005·Applied and Environmental Microbiology·Y AkutsuT Nakahara
Jan 20, 2006·Critical Reviews in Biotechnology·Ying ZhengAmarjeet S Bassi

❮ Previous
Next ❯

Citations

Apr 4, 2017·Microbial Biotechnology·Ren Wei, Wolfgang Zimmermann
Apr 16, 2020·Frontiers in Microbiology·María José Cárdenas EspinosaChristian Eberlein
Aug 9, 2020·Polymers·Aleksandra Kemona, Małgorzata Piotrowska
Feb 26, 2021·ChemSusChem·Audrey MagninLuc Avérous

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.