Isolation and characterization of an immortalized oral keratinocyte cell line of mouse origin.

Archives of Oral Biology
Neha ParikhLee Ann Garrett-Sinha

Abstract

To establish an oral epithelial cell line of mouse origin for molecular and biochemical assays. Epithelial cells were isolated from the oral cavity of adult mice and established as a spontaneously immortalized cell line in culture, designated immortalized oral keratinocyte cells (IMOK cells). The cells were then characterized for growth characteristics, differentiation potential, karyotype, transfectability, susceptibility to viral infection and responses to siRNA. The IMOK cells exhibit robust growth in both serum-containing and serum-free medium for at least 100 population doublings. IMOK cells have a near diploid karyotype, express keratinocyte marker proteins and can be induced to undergo differentiation by the addition of high levels of calcium to the medium. The differentiation process is characterized by morphological changes and by the induction of oral epithelium specific differentiation marker proteins such as K4 and K13. Transient transfection analyses reveal that IMOK cells are highly transfectable and that several promoters of epithelial cells are active in these cells. Moreover, upon differentiation with calcium, there is an up-regulation of differentiation-specific K4 and Elf5 promoter activity. Finally, we show ...Continue Reading

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Citations

Apr 3, 2012·Trends in Endocrinology and Metabolism : TEM·Heather J Lee, Christopher J Ormandy
Jul 15, 2015·Applied Microbiology and Biotechnology·Xing XieYongjie Liu
Aug 21, 2010·Journal of Biomedical Materials Research. Part a·Ji Suk Choi, Hyuk Sang Yoo
May 29, 2013·International Journal of Molecular Sciences·Manal FareaAdam Husein
May 23, 2021·Clinical and Experimental Dental Research·Fayrouz BazinaStephanos Kyrkanides

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