Isolation and characterization of an oxygen radical absorbance activity peptide from defatted peanut meal hydrolysate and its antioxidant properties

Journal of Agricultural and Food Chemistry
Lin ZhengMouming Zhao

Abstract

Defatted peanut meal hydrolysate (DPMH) was purified using ultrafiltration, gel filtration chromatography, and high-performance liquid chromatography. A tripeptide with strong oxygen radical absorbance capacity (ORAC) was isolated and identified as Tyr-Gly-Ser by ESI-MS/MS. It was then synthesized to measure its antioxidant properties in different systems. The ORAC value of Tyr-Gly-Ser was 3-fold higher than that of glutathione (GSH), and it displayed a stronger protective effect on linoleic acid peroxidation and H(2)O(2)-induced oxidative injury in rat pheochromocytoma line PC12 cells than GSH (p < 0.05). However, Tyr-Gly-Ser showed negligible DPPH radical scavenging activity, reducing power, and no metal chelating ability. The results suggested that Tyr-Gly-Ser displayed antioxidant activity via the hydrogen atom transfer mechanism, and the Tyr at the N-terminal was the hydrogen donor. The ORAC assay was recommended as a reliable and effective method to measure the antioxidant activity in the course of antioxidant peptide isolation.

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Citations

Jul 28, 2015·Food Chemistry·Mingzhu ZhuangGuowan Su
Nov 2, 2013·Food Chemistry·Adeola M AlashiRotimi E Aluko
Feb 19, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liying WangJingbo Liu
Apr 5, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lezaan PrinslooAnabella Gaspar
Jun 23, 2020·Journal of the Science of Food and Agriculture·Xuqing WeiXuepeng Li
Aug 9, 2019·Foods·Jelena ČakarevićLjiljana Popović
Oct 5, 2017·Journal of Food Science and Technology·Wenzhi LiGuowan Su
Apr 19, 2018·Journal of Food Science and Technology·Hesheng LiuZhida Sun
Sep 7, 2017·Food Research International·Pádraigín A HarnedyRichard J FitzGerald
Jun 19, 2021·Current Research in Food Science·Erwann DurandPierre Villeneuve

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