Isolation and characterization of an R-prime plasmid from Rhizobium meliloti.

Journal of Bacteriology
G B KissA Kondorosi

Abstract

Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria.

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Citations

Jan 1, 1980·Molecular & General Genetics : MGG·J CasadesúsJ Olivares
Jun 1, 1981·Genetical Research·P L Royle, B W Holloway
Jan 1, 1981·Molecular & General Genetics : MGG·Z BánfalviA Kondorosi
Feb 1, 1983·Journal of Bacteriology·T ForraiA Kondorosi
Jul 1, 1984·Journal of Bacteriology·M O Martin, S R Long

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