Jan 1, 1976

Isolation and characterization of an unsaturated fatty acid-requiring mutant of cultured mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
T Y Chang, P R Vagelos

Abstract

An unsaturated fatty acid-requiring mutant derived from Chinese hamster ovary (CHO) cells has been isolated and characterized. This mutant grows normally when oleate or other unsaturated fatty acids are supplemented in the growth medium. Unlike the wild-type CHO cells, growth stops when medium is deprived of unsaturated fatty acid. Whole cell pulse experiments with [14C]acetate or [14C]stearate indicate that the mutant is defective in unsaturated fatty acid synthesis. Enzyme assays in vitro show that the enzymatic defect of the mutant is localized to the microsomal stearoyl-CoA desaturase.

  • References12
  • Citations14

References

Mentioned in this Paper

Ovarian Diseases
Chinese Hamster
Acyl CoA Desaturases
NADPH-Ferrihemoprotein Reductase
Both Ovaries
Microsomes
Ovarian Neoplasm
Ovary
Chinese Hamster Ovary Cell
Thyroid Hormone Plasma Membrane Transport Defect

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