Isolation and characterization of an anticoagulant from the salivary glands of the tick, Ornithodoros savignyi (Acari: Argasidae)

Experimental & Applied Acarology
A R M GasparAlbert W H Neitz

Abstract

An inhibitor of activated coagulation factor X (fXa) was isolated from salivary gland extracts prepared from Ornithodoros savignyi using a two-step procedure, involving reversed-phase high-performance liquid chromatography (RP-HPLC) and diethylaminoethyl (DEAE) ion-exchange chromatography. From its behaviour during DEAE chromatography it could be deduced that it possesses an acidic pI (approximately 4.6). Capillary zone electrophoresis (CZE) of the purified inhibitor showed it to be homogeneous. The molecular mass was determined as 12 kDa using capillary gel electrophoresis (CGE) and as 7183.4 using laser desorption mass spectrometry (LDMS). The N-terminal amino acid sequence (residues 1-12) was determined and found to share a 66% identity with tick anticoagulant peptide (TAP). The O. savignyi peptide is a slow, tight-binding inhibitor of fXa (Ki = 0.83 +/- 0.10 nM). The interaction of the fXa--inhibitor was found to be competitive and dependent on ionic strength. Preliminary investigations show that the inhibitor may be specific for fXa.

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Citations

Aug 22, 2007·Experimental & Applied Acarology·Clarisse Gravina RicciCarlos Termignoni
Jan 16, 2004·Insect Biochemistry and Molecular Biology·Ben J Mans, Albert W H Neitz
Nov 6, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·B J MansA W Neitz
Nov 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M A IbrahimM I Khalil
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Ivo M B FrancischettiJose M C Ribeiro
Feb 13, 2016·Ticks and Tick-borne Diseases·Ben J MansAbdalla A Latif
May 9, 2012·Journal of Proteomics·Jindrich ChmelarMichail Kotsyfakis
Jul 28, 2009·Insect Biochemistry and Molecular Biology·María Angeles Corral-RodríguezPablo Fuentes-Prior
Dec 12, 2007·Insect Biochemistry and Molecular Biology·Ivo M B FrancischettiJosé M C Ribeiro
Nov 6, 2004·Gene·Ren LaiPhilip C Turner
Dec 21, 2005·Toxicon : Official Journal of the International Society on Toxinology·N A SteenP F Alewood
Feb 15, 2008·PLoS Medicine·Joppe W R HoviusErol Fikrig
Dec 12, 2012·International Journal for Parasitology·Elizabeth LouwChristine Maritz-Olivier
Jul 28, 2007·Trends in Parasitology·Christine Maritz-OlivierAnabella R M Gaspar
Nov 8, 2017·Medical and Veterinary Entomology·L F PariziI DA Silva Vaz
Jun 9, 2005·Parasitology·A S Bowman, J R Sauer
Nov 8, 2002·Insect Molecular Biology·S NarasimhanE Fikrig
Jun 8, 2017·Frontiers in Cellular and Infection Microbiology·Adrien A BlisnickSarah I Bonnet
Dec 1, 2008·Expert Review of Hematology·Cho Yeow Koh, R Manjunatha Kini

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