Isolation and characterization of an oligodendrocyte precursor-derived B-cell epitope in multiple sclerosis

Annals of Neurology
J ArchelosH P Hartung

Abstract

In a search for possible central nervous system-specific autoantigens in multiple sclerosis (MS), a lambda-phage protein expression library was constructed from an oligodendrocyte-precursor cell line. The library was screened with pooled cerebrospinal fluid (CSF) from 54 patients with definite MS according to the criteria of Poser. Pooled CSF samples from 44 patients with other neurological diseases including bacterial meningitis and viral encephalitis were used as control. A total of 1,000,000 colonies were screened and 6 positive clones were detected. At the DNA level none of the sequences showed significant homology to a known coding sequence. All 6 clones contained an open reading frame for small peptides ranging from 14 to 38 amino acids. It was noteworthy that 5 clones contained a common sequence of 7 amino acids, which was highly homologous to a translated consensus Alu repeat epitope. Screening of sera and CSF from patients with MS showed that approximately 44% reacted with these so-called Alu peptides, end-point antibody titers in their sera ranging from 1:1,000 to 1:25,000. In addition, some samples selected by their reactivity with Alu peptides stained intensively the cytoplasm of oligodendrocyte precursors but not o...Continue Reading

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Citations

Feb 5, 1998·Annals of Neurology·H GarrenC Lock
Apr 15, 2003·Current Neurology and Neuroscience Reports·Bernhard HemmerHans-Peter Hartung
Mar 17, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Deena VardhiniLavanya M Suneetha
Dec 14, 2002·Journal of the Neurological Sciences·Permphan Dharmasaroja
Dec 8, 2000·Journal of Neuroimmunology·A H CrossJ Lyons
Sep 25, 1999·Journal of Neuroimmunology·F Lu, B Kalman
Jun 17, 2000·Trends in Neurosciences·J J Archelos, H P Hartung
Jul 13, 2012·The New England Journal of Medicine·Rajneesh SrivastavaBernhard Hemmer
Apr 21, 2005·The Journal of Clinical Investigation·Sabine CepokBernhard Hemmer
Aug 12, 2014·Autoimmunity Reviews·Judith FraussenVeerle Somers
Apr 28, 2009·Autoimmunity Reviews·Klaartje SomersVeerle Somers
Mar 21, 2007·Journal of the Neurological Sciences·Thomas Berger, Markus Reindl
Aug 29, 2006·Journal of Neuroimmunology·Markus ReindlThomas Berger
Nov 1, 2005·Journal of Autoimmunity·V SomersP Stinissen
Jan 28, 2015·Nature Reviews. Neurology·Panos StathopoulosMarinos C Dalakas
Sep 5, 2002·Nature Reviews. Neuroscience·Robin J M Franklin
Mar 5, 2014·Multiple Sclerosis : Clinical and Laboratory Research·Lucas SchirmerBernhard Hemmer
Dec 3, 1999·Brain : a Journal of Neurology·C LucchinettiH Lassmann
Apr 11, 2012·Current Opinion in Neurology·Tobias Derfuss, Edgar Meinl
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Veerle SomersPiet Stinissen
Nov 3, 2004·Autoimmunity·Thomas Berger
Sep 28, 1999·Current Opinion in Neurology·B C KieseierH P Hartung

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