PMID: 9658013Jul 11, 1998Paper

Isolation and characterization of Bacillus subtilis sigB operon mutations that suppress the loss of the negative regulator RsbX

Journal of Bacteriology
N SmirnovaW G Haldenwang

Abstract

sigmaB, a transcription factor that controls the Bacillus subtilis general stress response regulon, is activated by either a drop in intracellular ATP or exposure to environmental stress. RsbX, one of seven sigmaB regulators (Rsb proteins) whose genes are cotranscribed with sigmaB, is a negative regulator in the stress-dependent activation pathway. To better define the interactions that take place among the Rsb proteins, we analyzed sigB operon mutations which suppress the high-level sigmaB activity that normally accompanies the loss of RsbX. Each of these mutations was in one of three genes (rsbT, -U, and -V) which encode positive regulators of sigmaB, and they all defined amino acid changes which either compromised the activities of the mutant Rsbs or affected their ability to accumulate. sigmaB activity remained inducible by ethanol in several of the RsbX- suppressor strains. This finding supports the notion that RsbX is not needed as the target for sigmaB activation by at least some stresses. sigmaB activity in several RsbX- strains with suppressor mutations in rsbT or -U was high during growth and underwent a continued, rather than a transient, increase following stress. Thus, RsbX is likely responsible for maintaining low...Continue Reading

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Citations

Dec 2, 2005·Journal of Molecular Microbiology and Biotechnology·Jan Pané-FarréJörg Stülke
Sep 3, 2003·Molecular Microbiology·Chien-Cheng ChenOlivier Delumeau
Jul 21, 2015·Photochemistry and Photobiology·Jeroen B van der Steen, Klaas J Hellingwerf
Jul 21, 2004·The Journal of Biological Chemistry·Olivier DelumeauRichard J Lewis
Dec 3, 2005·Journal of Bacteriology·Shuyu ZhangW G Haldenwang
Feb 27, 2004·Cell Stress & Chaperones·Wolfgang Schumann
Feb 23, 2020·International Journal of Molecular Sciences·Soujanya Lakshmi EgaSudhamani Muddada
Mar 27, 1999·Biochemical and Biophysical Research Communications·J M ScottW G Haldenwang
May 14, 1999·Current Opinion in Microbiology·J D Helmann
Apr 29, 2006·International Journal of Medical Microbiology : IJMM·Jan Pané-FarréMichael Hecker

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