Isolation and characterization of basal cells from human upper respiratory epithelium

Experimental Cell Research
W HicksJ Lwebuga-Mukasa

Abstract

Cellular pathways of normal and reparative differentiation of upper airway epithelium are not well understood. Of the three main cell types, basal and secretory cells are known to divide, while ciliated cells are considered terminally differentiated. Several investigations support the role of the basal cell as a progenitor cell type, but others suggest that the secretory cell can regenerate a complete mucocilliary epithelium. Thus, lineage relationships within renewing adult epithelia are still unclear. Understanding the pathways involved in upper airway epithelial cell differentiation is critical for studying injury and repair mechanisms and for developing clinical strategies for tracheal reconstruction. We undertook the current studies to determine the integrin profile of isolated human upper airway basal cells. Respiratory epithelial cells (REC) were isolated by elastase digestion, stained with FITC-labeled Griffonia simplicifolia isolectin B4 (GSI-B4), and sorted by flow cytometry. Approximately 80% of the lectin-positive cells were basal cells, as determined by morphology and cytokeratin staining. These cells expressed integrins alpha 1, alpha 2, alpha 3, alpha 5, alpha v beta 5, beta 1, beta 3, and alpha 6 beta 4, by immu...Continue Reading

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Citations

Jan 8, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Darryl A Knight, Stephen T Holgate
Aug 16, 2006·American Journal of Respiratory and Critical Care Medicine·Anthony KicicStephen M Stick
Oct 23, 2001·Respiratory Research·E Puchelle, B Peault
May 17, 2011·PloS One·Neil R HackettRonald G Crystal
Sep 17, 2004·Acta Oto-laryngologica·Alessandra Rinaldo, Alfio Ferlito
Jan 4, 2008·Journal of Drug Targeting·Bram SlütterWim Jiskoot
Mar 1, 2005·The Annals of Thoracic Surgery·Emmanuel MartinodAlain F Carpentier
Nov 21, 2014·American Journal of Respiratory Cell and Molecular Biology·Xiaofang WuMary C Rose
Nov 25, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rommel S LanPeter J Henry

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