PMID: 3755527Jul 25, 1986Paper

Isolation and characterization of bovine and mouse terminal deoxynucleotidyltransferase cDNAs expressible in mammalian cells

Nucleic Acids Research
O KoiwaiK Arai

Abstract

We have isolated nearly full-length cDNA clones of terminal deoxynucleotidyltransferase (TdT) from calf thymus and mouse lymphoma cDNA libraries. The libraries were constructed using the pcD vector system which permits the expression of cDNA inserts in mammalian cells. The bovine TdT cDNA clone contains an open reading frame coding for 520 amino acids, Mr 59,678. The mouse TdT cDNA clone contains an open reading frame of 1,587 bp, whose translated cDNA encodes a 60,004 dalton protein. The mouse TdT cDNA clone contains 60 bp in the 3' end region of the coding sequence not found in the bovine TdT cDNA sequence, otherwise, the clones share about 80% homology. A possible nuclear-localization-sequence (Pro-Arg-Lys-Lys-Arg-Pro-Arg) was conserved in the N-terminal region in the mouse and bovine cDNA clones. Bovine and mouse cDNAs transfected into COS7 monkey fibroblasts directed the synthesis of enzymatically active protein of Mr 60,000 which was detected immunologically using polyclonal rabbit antibody against bovine TdT. Bovine TdT expressed in COS7 cells by nearly full-length cDNA clone was localized in the nucleus and the translational product of pOK103 lacking the nuclear-localization-sequence was localized in the cytoplasm.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K E GregoireF J Bollum
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R C PetersonF J Bollum
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N R LandauD Baltimore
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F W Alt, D Baltimore
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T YokotaK Arai

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Citations

Apr 26, 1995·Journal of Immunological Methods·W HarrimanM Wabl
Jul 1, 1988·Mutation Research·B D Preston, L A Loeb
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M BogueD Mathis
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S KallenbachF Rougeon
Aug 26, 1998·The Journal of Experimental Medicine·C ThéveninM Busslinger
Aug 11, 1991·Nucleic Acids Research·J Ito, D K Braithwaite
Jun 11, 1995·Nucleic Acids Research·B YangM S Coleman
Jul 1, 1988·Journal of Chromatography·V N Pandey, M J Modak
Jul 15, 2009·Biochimica Et Biophysica Acta·Edward A Motea, Anthony J Berdis
Oct 31, 1989·Biochemical and Biophysical Research Communications·O KoiwaiY Yamada
Apr 1, 1989·Japanese Journal of Cancer Research : Gann·K OiwaT Kaneda
Apr 14, 1987·Biochemical and Biophysical Research Communications·O KoiwaiR Morishita
Jul 15, 1988·Biochemical and Biophysical Research Communications·O Koiwai, A Morita
Sep 14, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S IbeO Koiwai
Jun 25, 2003·Neuroreport·Sandra Peña De OrtizYuri I Arshavsky
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·To-Ha Thai, John F Kearney
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jamie A E RepaskyDavid G Schatz

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