Isolation and characterization of cDNA encoding three dehydrins expressed during Coffea canephora (Robusta) grain development

Annals of Botany
Cécile HinnigerJames McCarthy

Abstract

Dehydrins, or group 2 late embryogenic abundant proteins (LEA), are hydrophilic Gly-rich proteins that are induced in vegetative tissues in response to dehydration, elevated salt, and low temperature, in addition to being expressed during the late stages of seed maturation. With the aim of characterizing and studying genes involved in osmotic stress tolerance in coffee, several full-length cDNA-encoding dehydrins (CcDH1, CcDH2 and CcDH3) and an LEA protein (CcLEA1) from Coffea canephora (robusta) were isolated and characterized. The protein sequences deduced from the full-length cDNA were analysed to classify each dehydrin/LEA gene product and RT-PCR was used to determine the expression pattern of all four genes during pericarp and grain development, and in several other tissues of C. arabica and C. canephora. Primer-assisted genome walking was used to isolate the promoter region of the grain specific dehydrin gene (CcDH2). The CcDH1 and CcDH2 genes encode Y(3)SK(2) dehydrins and the CcDH3 gene encodes an SK(3) dehydrin. CcDH1 and CcDH2 are expressed during the final stages of arabica and robusta grain development, but only the CcDH1 transcripts are clearly detected in other tissues such as pericarp, leaves and flowers. CcDH3 t...Continue Reading

References

Jun 1, 1990·The Plant Cell·K Skriver, J Mundy
Jan 1, 1989·Molecular & General Genetics : MGG·A ShirsatD Boulter
Jul 31, 1999·Plant & Cell Physiology·T MatsuyamaT Hashimoto
Oct 4, 2000·Molecular & General Genetics : MGG·B ZhuT J Close
Jan 17, 2003·Plant Physiology·Myong-Chul KoagTimothy J Close
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Joseph G DubouzetKazuko Yamaguchi-Shinozaki
Aug 15, 2003·The Journal of Biological Chemistry·Muath K AlsheikhStephen K Randall
Nov 11, 2003·Biochemistry. Biokhimii︠a︡·Ch R AllagulovaV A Vakhitov
Jan 20, 2004·Trends in Plant Science·Michael J Wise, Alan Tunnacliffe
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·J. Ingram, D. Bartels
Jun 12, 2004·Plant Physiology and Biochemistry : PPB·Malinee ChatthaiSantosh Misra
Sep 10, 2004·Plant Molecular Biology·Tuula PuhakainenE Tapio Palva
Nov 8, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chenwei LinSteven D Tanksley
Jul 1, 1996·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D CrouzillatP Fritz

❮ Previous
Next ❯

Citations

Sep 13, 2012·Biotechnology Research International·M K Mishra, A Slater
Jan 7, 2014·Plant Signaling & Behavior·Irina I Vaseva, Urs Feller
Nov 13, 2008·Molecular Biology Reports·Dominique MingeotBernard Watillon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.