Isolation and characterization of circulating micro(nano)vesicles in the plasma of colorectal cancer patients and their interactions with tumor cells

Oncology Reports
Małgorzata StecMarek Zembala

Abstract

Micro(nano)vesicles (MV) are regarded as important messengers in cell-to-cell communication. There is also evidence for their pivotal role in cancer progression. Circulating MV are of different body cells origin, including tumor cell‑derived MV (TMV) in cancer patients. Determination of circulating TMV is of importance because of their potential diagnostic and therapeutic applications. In the present study, an analysis of circulating MV in colorectal cancer (CRC) patients was undertaken. Plasma from healthy donors was used as the control. In order to define MV characteristics, two plasma fractions: obtained by sequential centrifugation at 15,000 x g (MV15) and 50,000 x g (MV50) were used for analysis. The two fractions possessed a large range of sizes: 70(80)-1,300(1,400) nm and the most common particles with sizes 70-90 nm, both in patients and controls. Atomic force microscopy images of MV50 revealed a heterogeneous population of particles with different shapes and sizes. MV15 contained an increased level of CD41+ and CD61+ particles, suggesting their platelet origin. No difference between patients and controls was observed. A more precise analysis of MV50 showed the increased level of particles expressing EGFR (HER-1/Erb B1)...Continue Reading

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Citations

Aug 23, 2020·International Journal of Molecular Sciences·Wioletta OlejarzTomasz Lorenc
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Xuewu ZhangWenjuan Yu
Mar 21, 2017·Clinical & Experimental Metastasis·Magdalena SurmanMałgorzata Przybyło
Aug 1, 2020·International Journal of Molecular Sciences·Kerstin MenckClaudia Binder
Jun 26, 2020·Pathology Oncology Research : POR·Marek Z WojtukiewiczKenneth V Honn
Dec 4, 2021·Frontiers in Cell and Developmental Biology·Shaoshan Mai, Iwona Inkielewicz-Stepniak

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