Isolation and Characterization of Clostridium butyricum DSM 5431 Mutants with Increased Resistance to 1,3-Propanediol and Altered Production of Acids.

Applied and Environmental Microbiology
S Abbad-AndaloussiH Petitdemange

Abstract

Clostridium butyricum mutants were isolated from the parent strain DSM 5431 after mutagenesis with N-methyl-N(prm1)-nitro-N-nitrosoguanidine and two selection procedures: osmotic pressure and the proton suicide method. Isolated mutants were more resistant to glycerol and to 1,3-propanediol (1,3-PD) than was the wild type, and they produced more biomass. In batch culture on 62 g of glycerol per liter, the wild type produced more acetic acid than butyrate, with an acetate/butyrate ratio of 5.0, whereas the mutants produced almost the same quantities of both acids or more butyrate than acetate with acetate/butyrate ratios from 0.6 to 1.1. The total acid formation was higher in the wild-type strain. Results of analysis of key metabolic enzymatic activities were in accordance with the pattern of fermentation product formation: either the butyrate kinase activity increased or the acetate kinase activity decreased in cell extracts of the mutants. A decreased level of the hydrogenase and NADH-ferredoxin activities concomitant with an increase in ferredoxin-NAD(sup+) reductase activities supports the conclusion that the maximum percentage of NADH available and used for the formation of 1,3-PD was higher for the mutants (97 to 100%) than...Continue Reading

References

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Citations

Apr 10, 2010·Journal of Industrial Microbiology & Biotechnology·Yu-Tze HorngPo-Chi Soo
Mar 30, 2012·Applied Microbiology and Biotechnology·Afroditi ChatzifragkouSeraphim Papanikolaou
May 12, 2012·Applied Biochemistry and Biotechnology·Guneet KaurSubhash Chand
Nov 9, 2010·Biotechnology Journal·Hongjuan LiuDehua Liu
Aug 8, 2008·Journal of Industrial Microbiology & Biotechnology·Jian HaoDehua Liu
Oct 27, 2009·Applied and Environmental Microbiology·Burkhard OtteStefan Jennewein
Jul 31, 2013·Bioprocess and Biosystems Engineering·Fatma Gizem AvciNuri Azbar
Dec 20, 2014·Applied Microbiology and Biotechnology·Daria Szymanowska-Powałowska, Piotr Kubiak
Jan 11, 2001·Biochimica Et Biophysica Acta·G ChotaniK Sanford
Sep 1, 1996·Applied and Environmental Microbiology·S Abbad-AndaloussiH Petitdemange
Aug 13, 2005·Metabolic Engineering·María González-PajueloPhilippe Soucaille
Aug 12, 2009·Biotechnology Advances·R K SaxenaJasmine Isar
Apr 7, 2010·Biotechnology Advances·E Celińska

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