PMID: 6161639Oct 28, 1980Paper

Isolation and characterization of complementary deoxyribonucleic acid complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid from oocytes of Drosophila melanogaster

Biochemistry
C M WeideE S Goldstein

Abstract

The complementary deoxyribonucleic acid (cDNA) complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid [poly(A+) RNA] from Drosophila melanogaster oocytes has been isolated and characterized. Analysis of the kinetics of hybridization of this cDNA (cDNAHA) to total poly(A+) RNA of oocytes indicates this class contains approximately 86 different sequences. Hybridization kinetics of cDNAHA annealed to poly(A+) RNA from 19-old embryos is essentially the same as that of oocyte poly(A+) RNA. This suggests the highly abundant class of poly(A+) RNA persists in approximately the same frequency through early development. Analysis of the hybridization of cDNAHA to genomic DNA suggests that the highly abundant poly(A+) RNA from oocytes is not enriched for transcripts from repetitive sequences of the genome.

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·D J FinneganD S Hogness
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·Y V IlyinG P Georgiev
Jun 1, 1979·Experimental Cell Research·C G ArthurE S Goldstein
May 1, 1979·Developmental Biology·K V Anderson, J A Lengyel
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y KaufmannS Penman
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Apr 1, 1967·The Journal of Experimental Zoology·W W Doane

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Citations

Aug 22, 1986·Biochimica Et Biophysica Acta·E S GoldsteinK A Schultz

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