Jun 1, 1976

Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho

Proceedings of the National Academy of Sciences of the United States of America
A DasS Adhya


Polarity suppressor mutants that are conditional lethal for growth have been isolated in E. coli K12. The mutations map between the ilv and cya loci of the E. coli chromosome. Rho factor isolated from one of these ts mutants does not show transcription termination activity at any temperature tested; however, it is found to be temperature sensitive for its poly(C)-dependent ATPase activity. Unlike the previously known polarity suppressor mutants (suA and psu), the rho mutation suppresses all types of polarity. Other interesting properties of these mutants include ultraviolet sensitivity, recombination deficiency, and decreased ability to lysogenize temperate phages lambda and P1. Our results suggest that rho has an essential function in the growth and normal physiology of cells. The rho(ts) mutant allows the growth of phage lambda defective in the N gene. This result supports the model that N gene product prevents transcription termination by antagonizing rho activity.

Mentioned in this Paper

Alkalescens-Dispar Group
Transcription, Genetic
Recombination, Genetic
Effects of Radiation
Genome Mapping
DNA-dependent ATPase

About this Paper

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