PMID: 6991868Jan 1, 1980Paper

Isolation and characterization of context mutations affecting the suppressibility of nonsense mutations

Molecular & General Genetics : MGG
M M Fluck, R H Epstein

Abstract

Secondary mutations which increase the efficiency of suppression of nonsense mutations in the rIIB cistron of bacteriophage T4 have been isolated. These secondary mutations, called context mutations, map at sites very close to the nonsense codon, possibly on the promotor distal side. In context-nonsense double mutants, the amount of suppressed gene product is increased approximately 10-fold. The context mutations examined can act on the UAA (ochre) nonsense allele as well as on the UAG (amber) nonsense allele at a given site. These context mutations affect all suppression mechanisms analyzed (genetic suppressors, 5-fluorouracil suppression and spontaneous suppression). We suggest that context mutations affect information which is significant to the termination of polypeptide chains. According to our view, context mutations change the immediate neighborhood of nonsense mutations and so reduce the degree of resemblance to the sequences normally used for the termination of translation.

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D BelinR H Epstein
Mar 7, 1977·Molecular & General Genetics : MGG·M M FluckR H Epstein
Dec 15, 1977·Journal of Molecular Biology·C Coulondre, J H Miller
Dec 30, 1975·Molecular & General Genetics : MGG·E R Katz, S Brenner
Apr 1, 1972·Genetical Research·R Freedman, S Brenner
Jan 1, 1970·Advances in Genetics·G Mosig
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·W SalserR Epstein
Apr 29, 1967·Nature·J F SambrookS Brenner
Jul 14, 1967·Journal of Molecular Biology·A Sarabhai, S Brenner
Oct 13, 1969·Molecular & General Genetics : MGG·W Salser
Aug 28, 1970·Journal of Molecular Biology·A Srabhai, H Lamfrom
Apr 1, 1966·Journal of Molecular Biology·M Sekiguchi
Apr 1, 1962·Journal of Molecular Biology·S P CHAMPE, S BENZER
Apr 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·S P CHAMPE, S BENZER
Dec 30, 1961·Nature·F H CRICKR J WATTS-TOBIN
Mar 1, 1961·Proceedings of the National Academy of Sciences of the United States of America·S Benzer

❮ Previous
Next ❯

Citations

Jan 1, 1984·Molecular & General Genetics : MGG·T G Hagervall, G R Björk
Nov 1, 1986·Molecular & General Genetics : MGG·L A Kirsebom, L A Isaksson
Feb 25, 1981·Nucleic Acids Research·H Engelberg-Kulka
Jan 1, 1983·Molecular & General Genetics : MGG·M M Hryniewicz, R A Vonder Haar
Feb 15, 1983·Journal of Molecular Biology·J H Miller, A M Albertini
May 5, 1984·Journal of Molecular Biology·E J MurgolaK A Hijazi
Dec 1, 1990·Experientia·R H Buckingham
Jun 15, 1981·Journal of Molecular Biology·E J Murgola
May 20, 1992·Journal of Molecular Biology·J KopelowitzH Engelberg-Kulka
Oct 1, 2021·Nature Communications·Erika A DeBenedictisAhmed H Badran

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.