PMID: 2495980Apr 1, 1989Paper

Isolation and characterization of crinosomes--a subclass of secondary lysosomes

Experimental and Molecular Pathology
H GlaumannA M Motakefi

Abstract

A technique is presented for isolating a subclass of lysosomes, designated crinosomes, by one-step floating-up centrifugation in a cesium-containing sucrose gradient. Rats were treated with vinblastine in order to induce the formation of crinosomes. Vinblastine blocked exocytosis of secretory granules at the cell border. Later on, lipoprotein-containing granules were seen throughout the cytoplasm. Immunolabeling with polyclonal antibodies against albumin demonstrated a severalfold greater presence of gold particles over crinosomes and Golgi cisternae than over the surrounding organelles. The induction of crinosomes by vinblastine made it possible to isolate thenm on a sucrose gradient. These organelles contained marker enzymes for the Golgi complex (galactosyl transferase) and lysosomes (cathepsins). The purity of the fraction was high. The crinosomes were proteolytically and lipolytically very active. The crinosomes were positive to cytochemical acid phosphatase staining. It is concluded that crinosomes develop from fusion between lysosomes and secretory granules and that they are active in degrading retained secretory material.

References

Feb 1, 1975·The Journal of Cell Biology·H GlaumannJ L Ericsson
Jul 1, 1975·The Journal of Cell Biology·C M RedmanG E Palade
Oct 1, 1973·The Journal of Cell Biology·J J BergeronG E Palade
Oct 1, 1970·The Journal of Cell Biology·H Glaumann, G Dallner
Aug 1, 1959·The Journal of Biophysical and Biochemical Cytology·A B NOVIKOFF

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Citations

Feb 4, 2006·Neurobiology of Aging·Sabrina S Seehafer, David A Pearce
Oct 27, 2017·The Journal of Cell Biology·Tamás CsizmadiaGábor Juhász

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