PMID: 12766046May 27, 2003Paper

Isolation and characterization of cultured human conjunctival goblet cells

Investigative Ophthalmology & Visual Science
Marie A ShatosDarlene A Dartt

Abstract

To isolate and characterize goblet cells from normal human conjunctival tissue to determine whether epidermal growth factor (EGF) receptors are present and whether EGF can influence goblet cell proliferation. Goblet cells were isolated from explant cultures established from normal conjunctival tissue harvested from patients during periocular surgery. The cells were grown in RPMI culture medium supplemented with 10% fetal bovine serum and characterized using morphology, histochemistry, indirect immunofluorescence microscopy, molecular biology, and biochemistry. Proliferation was determined with a MTT proliferation assay after exposing goblet cells, which had been serum deprived for 48 hours, to increasing concentrations of epidermal growth factor (EGF; 0-80 ng/mL) for 24 hours. Goblet cells were isolated from conjunctival explants by scraping nongoblet cells from the culture dish. Human goblet cells exhibited positive reactivity with alcian blue-periodic acid Schiff (PAS) reagent, goblet cell-specific cytokeratin-7, HPA lectin, and MUC5AC, but negative reactivity to the stratified squamous epithelial cell marker, cytokeratin-4. The mRNA for MUC5AC was detected using RT-PCR. The presence of the EGF receptors EGFR, ErbB2, and ErbB...Continue Reading

Citations

May 17, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Jae-hyung KimHungwon Tchah
Jan 20, 2004·Experimental Eye Research·Darlene A Dartt
Apr 27, 2004·Experimental Eye Research·James D ZieskeXiaoqing Guo
Apr 19, 2008·Investigative Ophthalmology & Visual Science·Marie A ShatosDarlene A Dartt
Sep 8, 2012·Investigative Ophthalmology & Visual Science·Dayu LiDarlene A Dartt
Nov 19, 2010·Regenerative Medicine·Stefan SchraderJulie T Daniels
Jan 24, 2014·Mediators of Inflammation·L Contreras-RuizS Masli
Jan 4, 2013·Current Eye Research·Daniel R SabanJerry Y Niederkorn
Sep 15, 2012·Experimental Eye Research·Robin R HodgesDarlene A Dartt
Jun 15, 2007·Experimental Eye Research·So-Hyang ChungKyoung Yul Seo
Jan 24, 2017·Journal of Tissue Engineering and Regenerative Medicine·Shivani KasbekarPaul Rooney
Jul 21, 2009·Journal of Cutaneous Pathology·Doina IvanAlexander J F Lazar
Mar 22, 2006·Ophthalmic Plastic and Reconstructive Surgery·Todd R SheplerBita Esmaeli
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Darlene A DarttCharles N Serhan
Jul 25, 2017·Scientific Reports·Qiaozhi LuJennifer H Elisseeff
Jun 13, 2015·Eye & Contact Lens·Laura García-PosadasYolanda Diebold
Oct 29, 2020·Investigative Ophthalmology & Visual Science·Seiichi Yokoo, Satoru Yamagami
Dec 19, 2020·Pharmaceutics·Laura García-Posadas, Yolanda Diebold
Nov 25, 2018·Progress in Retinal and Eye Research·Christophe BaudouinMarc Labetoulle
Apr 27, 2021·Frontiers in Medicine·Donald R CampbellChelsea M Magin
Aug 29, 2021·Pharmaceutics·Yolanda Diebold, Laura García-Posadas

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