PMID: 9003587Feb 1, 1997Paper

Isolation and Characterization of Dehalogenases from2,2-Dichloropropionate-Degrading Soil Bacteria

Current Microbiology
R SchwarzeF R J Schmidt

Abstract

Five bacterial strains were isolated from polluted soilscapable of degrading 2,2-dichloropropionate. In crude extracts, dehalogenaseactivity against haloacetates and longer-chained 2-haloalkanoic acids couldbe detected. Results from activity staining indicated that all bacterialstrains expressed a single dehalogenase. In further biochemicalcharacterization, two types of D,L-specific 2-haloalkanoic acid dehalogenaseswere described, which are different from each other not only in molecularweight and electrophoretic mobility, but also in sensitivity towards thiolreagents. Dehalogenases of these strains have been shown to be inducible andare catalyzing halide hydrolysis with inversion of product configuration.

Citations

May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thiau-Fu AngThean Chor Leow
Aug 7, 2003·Applied and Environmental Microbiology·Julian R Marchesi, Andrew J Weightman
Aug 5, 2000·Applied and Environmental Microbiology·J S Tsang, B C Pang
Apr 3, 2020·Environmental Science. Processes & Impacts·Colin J CunninghamCharles W Knapp
May 11, 2007·Archives of Biochemistry and Biophysics·Sanela KurtovicBengt Mannervik

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