PMID: 6166624Jul 1, 1981Paper

Isolation and characterization of desmosome-associated tonofilaments from rat intestinal brush border

The Journal of Cell Biology
W W FrankeE D Jarasch

Abstract

Epithelial cells of the small intestine, like those of other internal organs, contain intermediate-sized filaments immunologically related to epidermal prekeratin which are especially concentrated in the cell apex. Brush-order fractions were isolated from rat small intestine, and apical tonofilaments attached to desmosomal plaques and terminal web residues were prepared therefrom by extraction in high salt (1.5 M KCl) buffer and Triton X-100. The structure of these filaments was indistinguishable from that of epidermal tonofilaments and, as with epidermal prekeratin, filaments could be reconstituted from solubilized, denatured intestinal tonofilament protein. On SDS polyacrylamide gel electrophoresis of proteins of the extracted desmosome-tonofilament fractions, a number of typical brush-border proteins were absent or reduced, and enrichment of three major polypeptides of Mr 55,000, 48,000, and 40,000 was noted. On two-dimensional gel electrophoresis, the three enriched major polypeptides usually appeared as pairs of isoelectric variants, and the two smaller components (Mr 48,000, and 40,000) were relatively acidic (isoelectric pH values of 5.40 and below), compared to the Mr 55,000 protein which focused at pH values higher tha...Continue Reading

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May 1, 1994·Cell and Tissue Research·A GebertR Pabst
Jan 1, 1989·Electron Microscopy Reviews·S W FrenchS H Swierenga
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J K FranzW W Franke
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Jan 1, 1988·Cell Motility and the Cytoskeleton·N M SawtellJ L Lessard
Jan 1, 1983·Differentiation; Research in Biological Diversity·R MollW W Franke
Jan 1, 1982·Differentiation; Research in Biological Diversity·W W FrankeH Mueller

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