Isolation and characterization of dexamethasone-resistant mutants from human lymphoid cell line CEM-C7.

Molecular and Cellular Biology
J M Harmon, E B Thompson

Abstract

Fifty-four independent dexamethasone-resistant clones were isolated from the clonal, glucocorticoid-sensitive human leukemic T-cell line CEM-C7. Resistance to 1 microM dexamethasone was acquired spontaneously at a rate of 2.6 X 10(-5) per cell per generation as determined by fluctuation analysis. After mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), the phenotypic expression time for dexamethasone resistance was determined to be 3 days. Spontaneous acquisition of resistance to 0.1 mM 6-thioguanine appeared to occur at a much slower rate, 1.6 X 10(-6) per cell per generation. However, the expression time after MNNG mutagenesis for this resistant phenotype was greater than 11 days, suggesting that the different rates of acquisition for the two phenotypes measured by fluctuation analysis were the results of the disparate expression times. The mutagens ICR 191 and MNNG were effective in increasing the dexamethasone-resistant fraction of cells in mutagenized cultures; ICR 191 produced a 35.6-fold increase, and MNNG produced an 8.5-fold increase. All the spontaneous dexamethasone-resistant clones contained glucocorticoid receptors, usually less than half of the amount found in the parental clone. They are therefore stri...Continue Reading

References

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Citations

Aug 1, 1985·Klinische Wochenschrift·E B ThompsonJ M Harmon
Nov 27, 1996·International Journal of Cancer. Journal International Du Cancer·M HalaR Kofler
Nov 5, 1997·The Journal of Biological Chemistry·H ChenE B Thompson
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·E Brad Thompson
Jun 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D G ChiltonE B Thompson
Apr 1, 1993·In Vitro Cellular & Developmental Biology. Animal·S KawaE B Thompson
Dec 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·Ingrid HerrMarkus W Büchler
Oct 20, 2001·Experimental Cell Research·M El-NaghyE B Thompson
Feb 28, 2001·The Journal of Steroid Biochemistry and Molecular Biology·F ZhouE B Thompson
Aug 23, 2002·Neoplasia : an International Journal for Oncology Research·Yan-shan JiE Brad Thompson
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·E B ThompsonB H Johnson
Jun 1, 1983·Journal of Steroid Biochemistry·H D Bradshaw, W V Vedeckis
Dec 1, 1986·Molecular and Cellular Endocrinology·U Gehring
Jan 1, 1990·Journal of Steroid Biochemistry·D V HarbourE B Thompson
Jan 1, 1986·Journal of Steroid Biochemistry·D J GruolS Bourgeois
Jan 1, 1987·Journal of Steroid Biochemistry·E B Thompson
Jan 1, 1983·Molecular and Cellular Endocrinology·R M GledhillM R Norman
Mar 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·S Ayala-TorresE B Thompson
Sep 1, 1994·Molecular and Cellular Biology·H Gourdeau, P R Walker
Feb 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D Duval, M Freyss-Beguin

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