PMID: 7536412Apr 1, 1995Paper

Isolation and characterization of distinct domains of sarcolemma and T-tubules from rat skeletal muscle

The Biochemical Journal
P MuñozA Zorzano

Abstract

1. Several cell-surface domains of sarcolemma and T-tubule from skeletal-muscle fibre were isolated and characterized. 2. A protocol of subcellular fractionation was set up that involved the sequential low- and high-speed homogenization of rat skeletal muscle followed by KCl washing, Ca2+ loading and sucrose-density-gradient centrifugation. This protocol led to the separation of cell-surface membranes from membranes enriched in sarcoplasmic reticulum and intracellular GLUT4-containing vesicles. 3. Agglutination of cell-surface membranes using wheat-germ agglutinin allowed the isolation of three distinct cell-surface membrane domains: sarcolemmal fraction 1 (SM1), sarcolemmal fraction 2 (SM2) and a T-tubule fraction enriched in protein tt28 and the alpha 2-component of dihydropyridine receptor. 4. Fractions SM1 and SM2 represented distinct sarcolemmal subcompartments based on different compositions of biochemical markers: SM2 was characterized by high levels of beta 1-integrin and dystrophin, and SM1 was enriched in beta 1-integrin but lacked dystrophin. 5. The caveolae-associated molecule caveolin was very abundant in SM1, SM2 and T-tubules, suggesting the presence of caveolae or caveolin-rich domains in these cell-surface memb...Continue Reading

Citations

Aug 4, 1998·The American Journal of Physiology·M ZhouK V Kandror
Apr 17, 2004·Traffic·Mhairi C TowlerFrances M Brodsky
Feb 28, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Valeria BarresiGaetano Barresi
Jan 30, 2008·Current Protocols in Cell Biology·Antonio Zorzano, Marta Camps
May 26, 2001·The Journal of Clinical Investigation·C YangJ E Pessin
Dec 25, 2009·The EMBO Journal·France Piétri-RouxelLuis Garcia
Feb 8, 2002·American Journal of Physiology. Cell Physiology·Dany SalvailEric Rousseau
Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·M AgoteF Escrivá
Jan 4, 2013·Skeletal Muscle·Jamie L Marshall, Rachelle H Crosbie-Watson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.