PMID: 9555889Apr 29, 1998Paper

Isolation and characterization of EPD1, an essential gene for pseudohyphal growth of a dimorphic yeast, Candida maltosa

Journal of Bacteriology
T NakazawaM Takagi

Abstract

Additional copies of the centromeric DNA (CEN) region induce pseudohyphal growth in a dimorphic yeast, Candida maltosa (T. Nakazawa, T. Motoyama, H. Horiuchi, A. Ohta, and M. Takagi, J. Bacteriol. 179:5030-5036, 1997). To understand the mechanism of this transition, we screened the gene library of C. maltosa for sequences which could suppress this morphological change. As a result, we isolated the 5' end of a new gene, EPD1 (for essential for pseudohyphal development), and then cloned the entire gene. The predicted amino acid sequence of Epdlp was highly homologous to those of Ggp1/Gas1/Cwh52p, a glycosylphosphatidylinositol-anchored protein of Saccharomyces cerevisiae, and Phr1p and Phr2p of Candida albicans. The expression of EPD1 was moderately regulated by environmental pH. A homozygous EPD1 null mutant showed some morphological defects and reduction in growth rate and reduced levels of both alkali-soluble and alkali-insoluble beta-glucans. Moreover, the mutant could not undergo the transition from yeast form to pseudohyphal form induced by additional copies of the CEN sequence at pH 4 or by n-hexadecane at pH 4 or pH 7, suggesting that EPD1 is not essential for yeast form growth but is essential for transition to the pseud...Continue Reading

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Citations

Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patricia BarralRosalía Rodríguez
Aug 10, 2001·Microbiology·M WeigF A Mühlschlegel
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Jun 28, 2000·The Journal of Biological Chemistry·T FontaineJ P Latgé
Jun 16, 2017·Expert Opinion on Investigational Drugs·Matthew W McCarthy, Thomas J Walsh
Aug 23, 2000·Yeast·D UccellettiC Palleschi
Jan 8, 1999·Biochimica Et Biophysica Acta·L Popolo, M Vai
Aug 25, 1999·Current Opinion in Microbiology·G J SmitsF M Klis
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Frances TrailH Corby Kistler

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