Isolation and characterization of exocellular polysaccharides produced by Bifidobacterium longum

Applied Microbiology and Biotechnology
S Abbad AndaloussiR Bonaly

Abstract

When grown anaerobically at pH values above 5.0, on ultrafiltered complex media containing excess lactose, Bifidobacterium longum formed up to 140 mg l-1 (glucose equiv.) exopolysaccharides. The highest yield was obtained when the cells were cultivated in a peptone/yeast extract medium with pH controlled by additions of NH4OH. Whatever the conditions under study, exopolysaccharides represented about 30% of the polysaccharides produced by B. longum after 48 h of culture. Crude pronase-treated exopolysaccharide preparations were adsorbed on ion-exchange chromatographic resin to yield an anionic heteropolysaccharide fraction. Two subfractions with apparent molecular masses of 1.2 MDa and 0.36 MDa respectively were subsequently recovered after gel filtration on Sepharose 4B. In both subfractions, glucose, galactose and small amounts of uronic acids and hexosamines were present in similar molar proportions, suggesting that the excreted polymers may be synthesized from the same base unit and may have a structure resulting from repeating subunits.

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Citations

Jun 18, 2011·Applied Microbiology and Biotechnology·Ilseon S JungIn Soo Kong
Jun 9, 2005·FEMS Microbiology Reviews·Adrianne KlijnFabrizio Arigoni
Jan 11, 2012·Journal of Agricultural and Food Chemistry·Nuria SalazarClara G de Los Reyes-Gavilán
Jul 19, 2002·Molecular Microbiology·Adam J Haugo, Paula I Watnick
Dec 1, 2012·Probiotics and Antimicrobial Proteins·Claudio Hidalgo-CantabranaPatricia Ruas-Madiedo
Feb 16, 2011·International Journal of Food Microbiology·Michelle CroninDouwe van Sinderen
Dec 19, 2002·Journal of Dairy Science·R M PigeonS E Gililliand
May 11, 1999·FEMS Microbiology Reviews·L De Vuyst, B Degeest

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