PMID: 7519849Aug 1, 1994Paper

Isolation and characterization of fibronectin-alpha 1-microglobulin complex in rat plasma

The Biochemical Journal
C FalkenbergB Akerström

Abstract

Molecules containing the 28 kDa immunoregulatory protein alpha 1-microglobulin (alpha 1-m), also known as protein HC, were isolated from rat plasma or serum by immunoaffinity chromatography. Three molecular species were distinguished on the basis of nondenaturing PAGE. Two of these have been described previously: uncomplexed alpha 1-m, and the complex of alpha 1-m with alpha 1-inhibitor-3. The third species was analysed by denaturing PAGE, immunoblotting, proteinase digestion and N-terminal-sequence analyses, and shown to consist of a complex between alpha 1-m and fibronectin. This complex, with a mass of about 560 kDa, was resistant to dissociation in the presence of denaturants, but not in the presence of reducing agents in combination with denaturants, and we conclude that the two components are linked by disulphide bonds. About 60% of the total detectable plasma alpha 1-m exists as high-molecular-mass complexes distributed approximately evenly between fibronectin and alpha 1-inhibitor-3. Immunochemical analyses were used to determine the proportion of the total plasma pools of fibronectin and alpha 1-inhibitor-3 that circulate in complex with alpha 1-m. About 3-7% of the total plasma fibronectin from three different rat str...Continue Reading

Citations

Jul 22, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T BerggårdJ J Enghild
Jan 31, 2002·Arthritis and Rheumatism·Peter OlofssonRikard Holmdahl
Apr 20, 2012·The Biochemical Journal·Winfried Meining, Arne Skerra
Nov 10, 2004·The Biochemical Journal·Jasjit S GrewalSaeed R Khan
Mar 31, 1999·American Journal of Reproductive Immunology : AJRI·T BerggårdB Akerström
Mar 23, 2021·Frontiers in Physiology·Jesper BergwikBo Åkerström
Nov 4, 2000·Biochimica Et Biophysica Acta·D R Flower
Nov 4, 2000·Biochimica Et Biophysica Acta·B AkerströmA Lindqvist
May 18, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K YoshidaH Sinohara
Jul 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joris Penders, Joris R Delanghe
Nov 16, 2004·Archives of Biochemistry and Biophysics·Jörgen LarssonBo Kerström
Mar 17, 2007·The Journal of Nutritional Biochemistry·Nanmei LiuMaria C Linder

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