Isolation and characterization of fin whale prolactin

International Journal of Peptide and Protein Research
H Kawauchi, M Tubokawa

Abstract

A highly purified prolactin preparation has been obtained from fin whale pituitaries by extraction with acid acetone. salt precipitation, isoelectric fractionation, and exclusion chromatography on Sephadex G-100 and ion-exchange chromatography on DEAE-CELLULOSE. Fin whale prolactin was isolated in a yield of 250 mg/kg wet weight tissue. It was found to have a molecular weight (SDS disc gel electrophoresis) of 23,600 daltons and an alpha-helix content (circular dichroism) of 50%. The amino acid composition and circular dichroism spectra were very similar to those of porcine prolactin. The partial amino acid sequence has been determined by the method of fluorescein-isothiocyanate. Fin whale prolactin was found to be 80% as potent as ovine prolactin with regard to pigeon crop-sac assay.

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Citations

Jan 16, 1984·European Journal of Biochemistry·K KohmotoF Sakiyama
Mar 1, 1989·General and Comparative Endocrinology·T KobayashiY Yokoo
Jan 1, 1984·General and Comparative Endocrinology·P Prunet, L M Houdebine
Jul 1, 1986·General and Comparative Endocrinology·K YamamotoS Kikuyama
Mar 1, 1983·General and Comparative Endocrinology·H KawauchiY Nakai
Aug 1, 1985·General and Comparative Endocrinology·T HiranoS Ishii
Aug 31, 1982·Biochemistry·T A BewleyF Talamantes

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