Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii

Plant Physiology
Q Chen, C D Silflow

Abstract

To elucidate the role of glutamine synthetase (GS) in nitrogen assimilation in the green alga Chlamydomonas reinhardtii we used maize GS1 (the cytosolic form) and GS2 (the chloroplastic form) cDNAs as hybridization probes to isolate C. reinhardtii cDNA clones. The amino acid sequences derived from the C. reinhardtii clones have extensive homology with GS enzymes from higher plants. A putative amino-terminal transit peptide encoded by the GS2 cDNA suggests that the protein localizes to the chloroplast. Genomic DNA blot analysis indicated that GS1 is encoded by a single gene, whereas two genomic fragments hybridized to the GS2 cDNA probe. All GS2 cDNA clones corresponded to only one of the two GS2 genomic sequences. We provide evidence that ammonium, nitrate, and light regulate GS transcript accumulation in green algae. Our results indicate that the level of GS1 transcripts is repressed by ammonium but induced by nitrate. The level of GS2 transcripts is not affected by ammonium or nitrate. Expression of both GS1 and GS2 genes is regulated by light, but perhaps through different mechanisms. Unlike in higher plants, no decreased level of GS2 transcripts was detected when cells were grown under conditions that repress photorespirati...Continue Reading

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Citations

Nov 8, 2012·Applied Microbiology and Biotechnology·Dong YanQingyu Wu
Nov 15, 2008·Journal of Experimental Botany·Chenping Xu, Bingru Huang
Aug 7, 2013·Journal of Experimental Botany·Emanuel Sanz-LuqueEmilio Fernandez
Apr 10, 2003·Eukaryotic Cell·Pushpa KathirCarolyn D Silflow
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Arthur Grossman, Hideki Takahashi
Sep 5, 2002·Physiologia Plantarum·Ritsuko InokuchiMitsumasa Okada
Aug 5, 2014·Molecular Biology Reports·Wei ZhaoQuan-Hong Yao
Dec 7, 2014·Plant Science : an International Journal of Experimental Plant Biology·Xiao WangBernd Wollenweber
Jul 27, 2004·The Plant Journal : for Cell and Molecular Biology·Josefa Gómez-MaldonadoMalcolm M Campbell
Apr 2, 2016·BioMed Research International·Aayudh DasJai S Rohila
Sep 9, 2006·Bioscience, Biotechnology, and Biochemistry·Masaru TerashitaKan Tanaka
Mar 26, 2002·Journal of Experimental Botany·Aurora GalvánEmilio Fernández
Nov 19, 2015·Frontiers in Plant Science·Emanuel Sanz-LuqueEmilio Fernandez
May 25, 2002·Chronobiology International·Ralf Werner
Jun 1, 2010·Journal of Proteome Research·Mei ChenFang Huang

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