Isolation and characterization of halophilic Archaea able to produce biosurfactants.

Journal of Industrial Microbiology & Biotechnology
S Kebbouche-GanaHocine Hacene

Abstract

Halotolerant microorganisms able to live in saline environments offer a multitude of actual or potential applications in various fields of biotechnology. This is why some strains of Halobacteria from an Algerian culture collection were screened for biosurfactant production in a standard medium using the qualitative drop-collapse test and emulsification activity assay. Five of the Halobacteria strains reduced the growth medium surface tension below 40 mN m(-1), and two of them exhibited high emulsion-stabilizing capacity. Diesel oil-in-water emulsions were stabilized over a broad range of conditions, from pH 2 to 11, with up to 35% sodium chloride or up to 25% ethanol in the aqueous phase. Emulsions were stable to three cycles of freezing and thawing. The components of the biosurfactant were determined; it contained sugar, protein and lipid. The two Halobacteria strains with enhanced biosurfactant producers, designated strain A21 and strain D21, were selected to identify by phenotypic, biochemical characteristics and by partial 16S rRNA gene sequencing. The strains have Mg(2+), and salt growth requirements are always above 15% (w/v) salts with an optimal concentration of 15-25%. Analyses of partial 16S rRNA gene sequences of the...Continue Reading

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Citations

Jun 12, 2013·Extremophiles : Life Under Extreme Conditions·Ikram DjeridiPhilippe Cuny
Aug 20, 2011·Journal of Industrial Microbiology & Biotechnology·Carol D Litchfield
Aug 21, 2013·Applied Biochemistry and Biotechnology·Arun Kumar PradhanBarada Kanta Mishra
Mar 5, 2014·Applied Biochemistry and Biotechnology·Pongsak NoparatAtipan Saimmai
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Sep 16, 2015·Extremophiles : Life Under Extreme Conditions·Débora NercessianRoberto Paggi
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