Isolation and characterization of herpes simplex virus type 1 mutants defective in the UL6 gene

Virology
A H PatelA J Davison

Abstract

Previous studies have shown that the protein encoded by herpes simplex virus type 1 (HSV-1) gene UL6 is required for processing and packaging of replicated viral DNA and is a minor component of virions and capsids. In this report, we describe the construction of UL6- HSV-1 mutants with a disrupted UL6 gene using complementing cells and show that they fail to synthesize the UL6 protein or produce infectious virus in noncomplementing cells. The mutants synthesized but failed to process and encapsidate viral DNA and accumulated only immature capsids which lacked the UL6 protein. Immunofluorescence analysis showed that the UL6 protein, when expressed transiently in transfected cells in the absence of other HSV-1 proteins, is localized exclusively to the nucleus. We also investigated an HSV-1 mutant with a defect in gene UL33, the product of which is also thought to be involved in viral DNA processing and packaging. The phenotype of this mutant on noncomplementing cells with regard to failure to process and encapsidate viral DNA, accumulation of immature capsids, and inability to produce infectious virus was the same as that of UL6- viruses. This mutant, however, produced capsids containing the UL6 protein, indicating that associati...Continue Reading

Citations

Sep 15, 2007·Journal of Virology·David G Meckes, John W Wills
Jan 1, 2010·Journal of Virology·Amy L HarperJohn W Wills
Dec 14, 2012·Journal of Virology·Sophia PavlovaHenri-Jacques Delecluse
Sep 2, 2011·Journal of Virology·Mindy LeelawongGregory A Smith
Jul 30, 2005·Journal of Virology·William W NewcombJay C Brown
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D G WolfE S Mocarski
Apr 6, 2012·Virus Research·Alexander J HowardRobert J Visalli
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