PMID: 4901359Nov 1, 1969

Isolation and characterization of host-independent Bdellovibrios

Journal of Bacteriology
R J Seidler, M P Starr

Abstract

A reliable method has been developed for the isolation of host-independent (H-I; i.e., "saprophytic") strains of Bdellovibrio from host-dependent (H-D; i.e., "parasitic") cultures. The technique involves growing streptomycin-resistant (Sm(r)) H-D cultures on streptomycin-susceptible (Sm(8)) host cells. A lysate containing large numbers of the Sm(r) H-D cells and some remaining Sm(8) host cells is transferred to a selection medium which contains the antibiotic. The Sm(8) host cells in the lysate are killed, and the Sm(r) H-I strains develop in broth within 3 to 6 days. By use of this method, it has been possible to isolate H-I strains from 16 different H-D Bdellovibrio strains studied. The frequency of occurrence of host independence is in the range of one H-I colony per 10(6) to 10(7) plaque-forming units of H-D bdellovibrios. The H-I cultures are nonfermentative, do not reduce nitrate, are strongly proteolytic, are oxidase-positive, and do not utilize 14 different carbon compounds as sources of energy for growth. Most H-I cultures are catalase-positive upon initial isolation from H-D lysates, but some cultures lose this enzyme upon subsequent transfers through host-free media. Most H-I bdellovibrios are pleomorphic, consisting...Continue Reading

Citations

Aug 26, 1977·Archives of Microbiology·M Eksztejn, M Varon
Feb 13, 1974·Archiv für Mikrobiologie·H M Engelking, R J Seidler
Jul 29, 2004·International Journal of Systematic and Evolutionary Microbiology·Marcie L BaerHenry N Williams
Sep 5, 2008·Current Protocols in Microbiology·Carey Lambert, Renee Elizabeth Sockett
Oct 22, 2008·Journal of Bacteriology·Susan R SteyertSilvia A Pineiro
Jul 7, 2009·Annual Review of Microbiology·Renee Elizabeth Sockett
Jan 1, 1988·Microbial Ecology·L E Casida
Jun 15, 2016·MicrobiologyOpen·Omotayo Opemipo OyedaraMario Alberto Rodriguez-Perez
Jul 21, 2005·Journal of Bacteriology·Susana CampoyJordi Barbé
Jul 16, 2008·Applied and Environmental Microbiology·John J TudorAdam Zwolak
Aug 10, 2012·Current Protocols in Microbiology·Edouard Jurkevitch
Mar 1, 2012·International Journal of Systematic and Evolutionary Microbiology·Susan F KovalEdouard Jurkevitch
Oct 19, 2010·Journal of Bacteriology·Alan J Wolfe
Feb 1, 1978·Archives of Microbiology·D Friedberg
Jul 4, 1974·Archives of Microbiology·V I TaylorR D Allen
Jan 1, 1973·Antonie van Leeuwenhoek·J C Huang, M P Starr
May 20, 2006·Nature·Francesca FiegnaGregory J Velicer
Aug 31, 2010·Applied and Environmental Microbiology·Andrew D MorganGregory J Velicer
Jul 31, 2007·Future Microbiology·Eckhard StrauchMichael Linscheid
Jan 1, 1989·Archives of Microbiology·K M Gray, E G Ruby
Feb 5, 1973·Archiv für Mikrobiologie·J C Huang, M P Starr
Jan 1, 1974·CRC Critical Reviews in Microbiology·M Varon
Sep 23, 2014·Applied and Environmental Microbiology·Megan A FergusonEileen M Spain
Dec 15, 2016·Frontiers in Microbiology·Łukasz MakowskiJolanta Zakrzewska-Czerwińska
Apr 10, 2007·Journal of Bacteriology·Katy J EvansRenee Elizabeth Sockett
Dec 1, 1975·Microbial Ecology·A MarbachM Shilo
Dec 22, 2009·Journal of Bacteriology·Andrew K FentonRenee Elizabeth Sockett
Nov 17, 2020·Frontiers in Microbiology·Henry N Williams, Huan Chen

Related Concepts

Bacteriological Techniques
Bacteriolysis
Vitreous Carbon
Cytochromes
Fermentation
Fungus Drug Sensitivity Tests
Electron Microscopy
Parasites
Peptide Hydrolases
Spectrometry

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