PMID: 2492179Jan 1, 1989Paper

Isolation and characterization of human fetal liver cytochrome P450HLp2: a third member of the P450III gene family

Archives of Biochemistry and Biophysics
S A Wrighton, M Vandenbranden

Abstract

We purified from human fetal livers a form of cytochrome P450, termed HLp2, related to adult human liver cytochrome P450HLp by monitoring the purification procedures by immunoblots developed with a monoclonal antibody to HLp. The preparation of HLp obtained was judged to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to have an apparent monomeric molecular weight slightly greater than that of HLp, 51,500 versus 51,000. Immunoblot and Ouchterlony double-diffusion analyses indicated that HLp and HLp2 are immunochemically related. However, ferrous-CO versus ferrous difference spectra yielded different Soret maxima for HLp and HLp2 (448.5 and 449.5 nm, respectively). In addition, structural comparisons between HLp and HLp2 indicated that they were distinct isozymes. Specifically, the 28 amino acid amino-terminal sequence determined for HLp2 was found to be 79% homologous to that of HLp. In addition, peptide mapping of HLp and HLp2 by limited proteolysis with three proteases yielded similar but different patterns indicating that HLp and HLp2 are structurally distinct. These results demonstrate that HLp2 and HLp are highly related but distinct proteins and that HLp2 is a member of the steroid-in...Continue Reading

References

May 15, 1988·Archives of Biochemistry and Biophysics·J R Halpert
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D T MolowaP S Guzelian
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P H BeauneF P Guengerich
Jan 1, 1986·CRC Critical Reviews in Biochemistry·M Adesnik, M Atchison
Jul 1, 1986·The Journal of Clinical Endocrinology and Metabolism·L MilewichB R Carr
Feb 1, 1987·DNA·D W NebertW Levin
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P B WatkinsP S Guzelian
Aug 15, 1985·Archives of Biochemistry and Biophysics·M KitadaY Kanakubo
Oct 22, 1970·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·S J YaffeS Orrenius
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack
Jul 1, 1980·Clinical Pharmacokinetics·M R JuchauC J Omiecinski

❮ Previous
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Citations

Jun 1, 1993·Journal of Pharmacological and Toxicological Methods·J L Raucy, S J Carpenter
Feb 9, 2000·Pharmacology & Therapeutics·J A RingD J Morgan
Dec 5, 1991·European Journal of Biochemistry·J M TreluyerT Cresteil
Dec 1, 1990·Risk Analysis : an Official Publication of the Society for Risk Analysis·N J Gorelick
Jul 1, 1998·Pharmacology & Toxicology·J HakkolaO Pelkonen
Aug 1, 1993·The Journal of Clinical Investigation·J D SchuetzP S Guzelian
Nov 6, 2007·Biological & Pharmaceutical Bulletin·Masataka MaruyamaShigeru Ohmori
Jan 25, 2006·Clinical Pharmacokinetics·Ann K Daly
Dec 17, 2002·Journal of Pharmacological and Toxicological Methods·J L FayerK J Ruterbories
Jun 6, 2008·Journal of Clinical Pharmacology·J Andrew WilliamsSteven A Wrighton
Feb 16, 2011·Paediatric Anaesthesia·Kathleen A NevilleGregory L Kearns
Feb 1, 1997·Pediatric Clinics of North America·J S Leeder, G L Kearns
Jan 4, 2001·Drug Metabolism Reviews·S A WrightonP B Watkins
Jan 1, 1993·Drug Metabolism Reviews·S A WrightonJ R Cashman
Jan 1, 1994·Drug Metabolism Reviews·M Kitada, T Kamataki
Aug 23, 2006·Expert Opinion on Drug Metabolism & Toxicology·Marc VermeirGeert Mannens
Aug 3, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Adaweyah El-MerhibiRoss A McKinnon
Jan 1, 1994·The Annals of Pharmacotherapy·L L Hoey, K D Lake
Jan 1, 1992·Critical Reviews in Toxicology·S A Wrighton, J C Stevens
Nov 1, 1989·Archives of Biochemistry and Biophysics·J D SchuetzP S Guzelian
Dec 31, 1997·Microscopy Research and Technique·K J Rich, A R Boobis
Dec 5, 1994·FEBS Letters·V CarrièreA Zweibaum
Jun 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D RatanasavanhA Guillouzo
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E G SchuetzP S Guzelian
Apr 1, 1991·Japanese Journal of Cancer Research : Gann·M KitadaT Kamataki
Sep 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Jeffrey C StevensMatthew J Zaya
Dec 1, 1995·Journal of Clinical Psychopharmacology·T A KetterA M Callahan
Jan 16, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S E Clarke
Jul 19, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Patrick J Murphy
Jan 16, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Bjarte FurnesDaniel Schlenk

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